Aims:TheaimofthisworkwastostudytheresponsesofSaccharomycesbayanuscellsexposedtosub-lethalhigh-pressurehomogenization(HPH)anddeterminewhethertheplasmaticmembranecansenseHPHinthepresence,orabsence,ofexogenousunsaturatedfattyacids(UFAs)inthegrowthmedium.MethodsandResults:High-pressurehomogenizationdamagedandcausedthecollapseofcellwallsandmembranesofaportionofcells;however,HPHdidnotsignificantlyaffectS.bayanuscellviability(lessthan0.3LogCFUml−1).HPHstronglyaffectedthemembranefattyacid(FA)compositionbyincreasingthepercentageoftotalUFAwhencomparedwithsaturatedfattyacids.ThegeneexpressionshowedthatthetranscriptionofOLE1,ERG3,andERG11increasedafterHPH.ThepresenceofexogenousUFAabolishedHPH- inducedeffectsontheOLE1andERG3genes,increasedthepercentageofmembranelipidsanddecreasedtheexpressionofOLE1andERG3within30minoftreatment.Conclusion:TheresultssuggestakeyroleforUFAinthemicrobialcellresponsetosub-lethalstress.Inaddition,thesedataprovideinsightintothemolecularbasisoftheresponseofS.bayanustothisinnovativetechnology.SignificanceandImpactoftheStudy:Elucidationofthemechanismofactionforsub-ethalHPHwillenabletheutilizationofthistechnologytomodulatethestarterperformanceattheindustrialscale.

Cell membrane fatty acid changes and desaturase expression of Saccharomyces bayanus exposed to high pressure homogenization in relation to the supplementation of exogenous unsaturated fatty acids

SERRAZANETTI, DIANA ISABELLA;PATRIGNANI, FRANCESCA;VANNINI, LUCIA;SIROLI, LORENZO;GARDINI, FAUSTO;LANCIOTTI, ROSALBA
2015

Abstract

Aims:TheaimofthisworkwastostudytheresponsesofSaccharomycesbayanuscellsexposedtosub-lethalhigh-pressurehomogenization(HPH)anddeterminewhethertheplasmaticmembranecansenseHPHinthepresence,orabsence,ofexogenousunsaturatedfattyacids(UFAs)inthegrowthmedium.MethodsandResults:High-pressurehomogenizationdamagedandcausedthecollapseofcellwallsandmembranesofaportionofcells;however,HPHdidnotsignificantlyaffectS.bayanuscellviability(lessthan0.3LogCFUml−1).HPHstronglyaffectedthemembranefattyacid(FA)compositionbyincreasingthepercentageoftotalUFAwhencomparedwithsaturatedfattyacids.ThegeneexpressionshowedthatthetranscriptionofOLE1,ERG3,andERG11increasedafterHPH.ThepresenceofexogenousUFAabolishedHPH- inducedeffectsontheOLE1andERG3genes,increasedthepercentageofmembranelipidsanddecreasedtheexpressionofOLE1andERG3within30minoftreatment.Conclusion:TheresultssuggestakeyroleforUFAinthemicrobialcellresponsetosub-lethalstress.Inaddition,thesedataprovideinsightintothemolecularbasisoftheresponseofS.bayanustothisinnovativetechnology.SignificanceandImpactoftheStudy:Elucidationofthemechanismofactionforsub-ethalHPHwillenabletheutilizationofthistechnologytomodulatethestarterperformanceattheindustrialscale.
Serrazanetti, Diana I.; Patrignani, Francesca; Russo, Alessandra; Vannini, Lucia; Siroli, Lorenzo; Gardini, Fausto; Lanciotti, Rosalba
File in questo prodotto:
File Dimensione Formato  
53_Serrazanetti et al 2015 Fr.pdf

accesso aperto

Tipo: Versione (PDF) editoriale
Licenza: Licenza per Accesso Aperto. Creative Commons Attribuzione (CCBY)
Dimensione 2.06 MB
Formato Adobe PDF
2.06 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/521198
Citazioni
  • ???jsp.display-item.citation.pmc??? 7
  • Scopus 15
  • ???jsp.display-item.citation.isi??? 15
social impact