Cell membrane fatty acid changes and desaturase expression of Saccharomyces bayanus exposed to high pressure homogenization in relation to the supplementation of exogenous unsaturated fatty acids

Serrazanetti, DIANA ISABELLA; Patrignani, Francesca; Russo, Alessandra; Vannini, Lucia; Siroli, Lorenzo; Gardini, Fausto; Lanciotti, Rosalba

doi:10.3389/fmicb.2015.01105

Aims:TheaimofthisworkwastostudytheresponsesofSaccharomycesbayanuscellsexposedtosub-lethalhigh-pressurehomogenization(HPH)anddeterminewhethertheplasmaticmembranecansenseHPHinthepresence,orabsence,ofexogenousunsaturatedfattyacids(UFAs)inthegrowthmedium.MethodsandResults:High-pressurehomogenizationdamagedandcausedthecollapseofcellwallsandmembranesofaportionofcells;however,HPHdidnotsignificantlyaffectS.bayanuscellviability(lessthan0.3LogCFUml−1).HPHstronglyaffectedthemembranefattyacid(FA)compositionbyincreasingthepercentageoftotalUFAwhencomparedwithsaturatedfattyacids.ThegeneexpressionshowedthatthetranscriptionofOLE1,ERG3,andERG11increasedafterHPH.ThepresenceofexogenousUFAabolishedHPH- inducedeffectsontheOLE1andERG3genes,increasedthepercentageofmembranelipidsanddecreasedtheexpressionofOLE1andERG3within30minoftreatment.Conclusion:TheresultssuggestakeyroleforUFAinthemicrobialcellresponsetosub-lethalstress.Inaddition,thesedataprovideinsightintothemolecularbasisoftheresponseofS.bayanustothisinnovativetechnology.SignificanceandImpactoftheStudy:Elucidationofthemechanismofactionforsub-ethalHPHwillenabletheutilizationofthistechnologytomodulatethestarterperformanceattheindustrialscale.

Serrazanetti, D.I., Patrignani, F., Russo, A., Vannini, L., Siroli, L., Gardini, F., et al. (2015). Cell membrane fatty acid changes and desaturase expression of Saccharomyces bayanus exposed to high pressure homogenization in relation to the supplementation of exogenous unsaturated fatty acids. FRONTIERS IN MICROBIOLOGY, 6(OCT), 1-10 [10.3389/fmicb.2015.01105].