Cell membrane fatty acid changes and desaturase expression of Saccharomyces bayanus exposed to high pressure homogenization in relation to the supplementation of exogenous unsaturated fatty acids

Serrazanetti, DIANA ISABELLA; Patrignani, Francesca; Russo, Alessandra; Vannini, Lucia; Siroli, Lorenzo; Gardini, Fausto; Lanciotti, Rosalba

doi:10.3389/fmicb.2015.01105

Aims:TheaimofthisworkwastostudytheresponsesofSaccharomycesbayanuscellsexposedtosub-lethalhigh-pressurehomogenization(HPH)anddeterminewhethertheplasmaticmembranecansenseHPHinthepresence,orabsence,ofexogenousunsaturatedfattyacids(UFAs)inthegrowthmedium.MethodsandResults:High-pressurehomogenizationdamagedandcausedthecollapseofcellwallsandmembranesofaportionofcells;however,HPHdidnotsignificantlyaffectS.bayanuscellviability(lessthan0.3LogCFUml−1).HPHstronglyaffectedthemembranefattyacid(FA)compositionbyincreasingthepercentageoftotalUFAwhencomparedwithsaturatedfattyacids.ThegeneexpressionshowedthatthetranscriptionofOLE1,ERG3,andERG11increasedafterHPH.ThepresenceofexogenousUFAabolishedHPH- inducedeffectsontheOLE1andERG3genes,increasedthepercentageofmembranelipidsanddecreasedtheexpressionofOLE1andERG3within30minoftreatment.Conclusion:TheresultssuggestakeyroleforUFAinthemicrobialcellresponsetosub-lethalstress.Inaddition,thesedataprovideinsightintothemolecularbasisoftheresponseofS.bayanustothisinnovativetechnology.SignificanceandImpactoftheStudy:Elucidationofthemechanismofactionforsub-ethalHPHwillenabletheutilizationofthistechnologytomodulatethestarterperformanceattheindustrialscale.

Cell membrane fatty acid changes and desaturase expression of Saccharomyces bayanus exposed to high pressure homogenization in relation to the supplementation of exogenous unsaturated fatty acids / Serrazanetti, Diana I.; Patrignani, Francesca; Russo, Alessandra; Vannini, Lucia; Siroli, Lorenzo; Gardini, Fausto; Lanciotti, Rosalba. - In: FRONTIERS IN MICROBIOLOGY. - ISSN 1664-302X. - STAMPA. - 6:OCT(2015), pp. 1105.1-1105.10. [10.3389/fmicb.2015.01105]