An improved high performance liquid chromatography (HPLC) method for the analysis of the main organic compounds in musts and wines is presented. A column packed with hydrogen sulfonated divinyl benzene-styrene copolymer and two detectors connected in series were used (UV at 210 nm and RI, respectively). The addition of acetonitrile (6%) to the mobile phase (0.045N H2SO4) allowed the simultaneous separation and quantification of several organic acids, glucose, fructose, glycerol, and ethanol. Direct injection and sample clean-up with a SAX cartridge was tested and compared. The SAX fractionation gave satisfactory results, however the direct injection of diluted wine (1:20) provided the best precision (CV ≤ 2.1%) and accuracy of analysis. Statistical analysis (paired t- test) disclosed significant differences only for glucose, fructose, α-ketoglutaric, and pyruvic acid. The role of column temperature for the analysis of vitamin C (ascorbic acid) was also investigated. Vitamin C underwent thermal degradation during analysis with column temperature ≥30°C.
An improved HPLC method for the analysis of organic acids, carbohydrates, and alcohols in grape musts and wines / Castellari M.; Versari A.; Spinabelli U.; Galassi S.; Amati A.. - In: JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES. - ISSN 1082-6076. - STAMPA. - 23:13(2000), pp. 2047-2056. [10.1081/JLC-100100472]
An improved HPLC method for the analysis of organic acids, carbohydrates, and alcohols in grape musts and wines
Versari A.
Membro del Collaboration Group
;Spinabelli U.Membro del Collaboration Group
;Galassi S.Membro del Collaboration Group
;Amati A.Membro del Collaboration Group
2000
Abstract
An improved high performance liquid chromatography (HPLC) method for the analysis of the main organic compounds in musts and wines is presented. A column packed with hydrogen sulfonated divinyl benzene-styrene copolymer and two detectors connected in series were used (UV at 210 nm and RI, respectively). The addition of acetonitrile (6%) to the mobile phase (0.045N H2SO4) allowed the simultaneous separation and quantification of several organic acids, glucose, fructose, glycerol, and ethanol. Direct injection and sample clean-up with a SAX cartridge was tested and compared. The SAX fractionation gave satisfactory results, however the direct injection of diluted wine (1:20) provided the best precision (CV ≤ 2.1%) and accuracy of analysis. Statistical analysis (paired t- test) disclosed significant differences only for glucose, fructose, α-ketoglutaric, and pyruvic acid. The role of column temperature for the analysis of vitamin C (ascorbic acid) was also investigated. Vitamin C underwent thermal degradation during analysis with column temperature ≥30°C.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
