Coenzyme Q10 (CoQ, ubiquinone) is a redox-active lipid endogenously synthesized by the cells. The final stage of CoQ biosynthesis is performed at the mitochondrial level by the "complex Q", where coq2 is responsible for the prenylation of the benzoquinone ring of the molecule. We report that the competitive coq2 inhibitor 4-nitrobenzoate (4-NB) decreased the cellular CoQ content and caused severe impairment of mitochondrial function in the T67 human glioma cell line. In parallel with the reduction of CoQ biosynthesis, the cholesterol level increased, leading to significant perturbation of the plasma membrane physico-chemical properties. We show that 4-NB treatment did not significantly affect the cell viability, because of an adaptive metabolic rewiring toward glycolysis. HIF-1α stabilization was detected in 4-NB treated cells, possibly due to the contribution of both reduction of intracellular oxygen tension and ROS overproduction. Exogenous CoQ supplementation partially recovered cholesterol content, HIF-1α degradation and ROS production, whereas only weakly improved the bioenergetic impairment induced by the CoQ depletion. Our data provide new insights on the effect of CoQ depletion and contribute to shed light on the pathogenic mechanisms of ubiquinone deficiency syndrome.

Coenzyme Q biosynthesis inhibition induces HIF-1α stabilization and metabolic switch toward glycolysis

Liparulo, Irene
Primo
;
Bergamini, Christian;Calonghi, Natalia;Gasparre, Giuseppe;Kurelac, Ivana;Rizzardi, Nicola;Rugolo, Michela;Wang, Wenping;Aleo, Serena J;Kiwan, Alisar;Torri, Cristian;Zanna, Claudia;Fato, Romana
Ultimo
2021

Abstract

Coenzyme Q10 (CoQ, ubiquinone) is a redox-active lipid endogenously synthesized by the cells. The final stage of CoQ biosynthesis is performed at the mitochondrial level by the "complex Q", where coq2 is responsible for the prenylation of the benzoquinone ring of the molecule. We report that the competitive coq2 inhibitor 4-nitrobenzoate (4-NB) decreased the cellular CoQ content and caused severe impairment of mitochondrial function in the T67 human glioma cell line. In parallel with the reduction of CoQ biosynthesis, the cholesterol level increased, leading to significant perturbation of the plasma membrane physico-chemical properties. We show that 4-NB treatment did not significantly affect the cell viability, because of an adaptive metabolic rewiring toward glycolysis. HIF-1α stabilization was detected in 4-NB treated cells, possibly due to the contribution of both reduction of intracellular oxygen tension and ROS overproduction. Exogenous CoQ supplementation partially recovered cholesterol content, HIF-1α degradation and ROS production, whereas only weakly improved the bioenergetic impairment induced by the CoQ depletion. Our data provide new insights on the effect of CoQ depletion and contribute to shed light on the pathogenic mechanisms of ubiquinone deficiency syndrome.
Liparulo, Irene; Bergamini, Christian; Bortolus, Marco; Calonghi, Natalia; Gasparre, Giuseppe; Kurelac, Ivana; Masin, Luca; Rizzardi, Nicola; Rugolo, Michela; Wang, Wenping; Aleo, Serena J; Kiwan, Alisar; Torri, Cristian; Zanna, Claudia; Fato, Romana
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11585/795346
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