Salad crops of the Brassicaceae family, such as Diplotaxis tenuifolia and Eruca vesicaria, commonly referred to as “rocket salads”, attracted considerable interest as culinary vegetables, for their strong flavour and the content of putative health-promoting compounds. Among these, glucosinolates and phenolics are well-known phytochemicals with an important role also in determining the characteristic flavour of these species. These two classes of components are therefore interesting for the evaluation of qualitative characters of germplasm of these two genera Within a program aimed at evaluating genetic resources of leafy vegetables, thirty-two Eruca and five Diplotaxis accessions, supplied from the IPK Genebank, Gatersleben, were studied by RP-HPLC-ESI-MS, in order to analyse their different polyphenols and glucosinolates profile. Glucosinolate profiles of the thirty-seven accessions were very similar, irrespective of the species, and ten desulpho(DS)-glucosinolates were detected in all extracts. Total GLS content ranged from 756 to 3031.1 mg/kg d.w. and the more abundant components were DS-glucoraphanin, DS-glucoerucin and the dimeric form of DS-glucosativin. Ample variability of phenolic profiles of D. tenuifolia and E. sativa was detected, corresponding to a prevalence of quercetin and kaempferol derivatives, respectively. The Eruca accessions exhibited very ample range of total flavonoid content, from 9.99 to 31.39 g/kg d.w., whereas Diplotaxis accessions ranged between 4.68 and 19.81 g/kg d.w. Differently to Diplotaxis, all the Eruca samples had kaempferol-3,4-diglucoside as the main flavonoid and it should be possible to identify this compound as a marker for the Eruca species. A principal component analysis highlighted the clear separation of the two genera on the basis of flavonoid profile, also revealing further, within-genus variability due to the association of specific components.

Evaluation of glucosinolates and phenolics in rocket salad (Eruca sativa and Diplotaxis tenuifolia) germplasm

PASINI, FEDERICA;VERARDO, VITO;CABONI, MARIA;D'ANTUONO, LUIGI FILIPPO
2011

Abstract

Salad crops of the Brassicaceae family, such as Diplotaxis tenuifolia and Eruca vesicaria, commonly referred to as “rocket salads”, attracted considerable interest as culinary vegetables, for their strong flavour and the content of putative health-promoting compounds. Among these, glucosinolates and phenolics are well-known phytochemicals with an important role also in determining the characteristic flavour of these species. These two classes of components are therefore interesting for the evaluation of qualitative characters of germplasm of these two genera Within a program aimed at evaluating genetic resources of leafy vegetables, thirty-two Eruca and five Diplotaxis accessions, supplied from the IPK Genebank, Gatersleben, were studied by RP-HPLC-ESI-MS, in order to analyse their different polyphenols and glucosinolates profile. Glucosinolate profiles of the thirty-seven accessions were very similar, irrespective of the species, and ten desulpho(DS)-glucosinolates were detected in all extracts. Total GLS content ranged from 756 to 3031.1 mg/kg d.w. and the more abundant components were DS-glucoraphanin, DS-glucoerucin and the dimeric form of DS-glucosativin. Ample variability of phenolic profiles of D. tenuifolia and E. sativa was detected, corresponding to a prevalence of quercetin and kaempferol derivatives, respectively. The Eruca accessions exhibited very ample range of total flavonoid content, from 9.99 to 31.39 g/kg d.w., whereas Diplotaxis accessions ranged between 4.68 and 19.81 g/kg d.w. Differently to Diplotaxis, all the Eruca samples had kaempferol-3,4-diglucoside as the main flavonoid and it should be possible to identify this compound as a marker for the Eruca species. A principal component analysis highlighted the clear separation of the two genera on the basis of flavonoid profile, also revealing further, within-genus variability due to the association of specific components.
2nd International Conference on FOOD-OMICS
93
94
F. Pasini; V. Verardo; M.F. Caboni; L.F. D’Antuono
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11585/103608
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