An HPLC method for the simultaneous quantification of the main carboxylic acids (including citric, tartaric, malic, shikimic, succinic, lactic, fumaric, and acetic acids), sugars (glucose and fructose), and alcohols (glycerol and ethanol) of musts and wine is proposed. Using a cation exchange column, improved separation of coeluting compounds (e.g., fructose vs. malic acid and succinic vs. shikimic acid) was achieved by moderate addition of tetrahydrofuran (THF) (4%) and n-propyl alcohol (n-PA) (5%) as organic modifier in the mobile phase. A factorial experiment was carried out for optimize acidity and temperature of elution. The method showed good linearity and precision and, as an application, was successfully applied to a must and a win

Simultaneous determination of organic acids, sugars, and alcohols in musts and wines by an improved ion-exclusion HPLC method / Fabio Chinnici, Umberto Spinabelli, Aureliano Amati. - In: JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES. - ISSN 1082-6076. - STAMPA. - 25:16(2002), pp. 2551-2560. [10.1081/JLC-120014274]

Simultaneous determination of organic acids, sugars, and alcohols in musts and wines by an improved ion-exclusion HPLC method

Fabio Chinnici
Primo
Writing – Original Draft Preparation
;
Umberto Spinabelli
Software
;
Aureliano Amati
Supervision
2002

Abstract

An HPLC method for the simultaneous quantification of the main carboxylic acids (including citric, tartaric, malic, shikimic, succinic, lactic, fumaric, and acetic acids), sugars (glucose and fructose), and alcohols (glycerol and ethanol) of musts and wine is proposed. Using a cation exchange column, improved separation of coeluting compounds (e.g., fructose vs. malic acid and succinic vs. shikimic acid) was achieved by moderate addition of tetrahydrofuran (THF) (4%) and n-propyl alcohol (n-PA) (5%) as organic modifier in the mobile phase. A factorial experiment was carried out for optimize acidity and temperature of elution. The method showed good linearity and precision and, as an application, was successfully applied to a must and a win
2002
Simultaneous determination of organic acids, sugars, and alcohols in musts and wines by an improved ion-exclusion HPLC method / Fabio Chinnici, Umberto Spinabelli, Aureliano Amati. - In: JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES. - ISSN 1082-6076. - STAMPA. - 25:16(2002), pp. 2551-2560. [10.1081/JLC-120014274]
Fabio Chinnici, Umberto Spinabelli, Aureliano Amati
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/903671
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