Two methods of analysis, micellar electrokinetic capillary chromatography (MECC) and reversed-phase high-performance liquid chromatography (RP-HPLC), were developed for the separation and determination of selected phenolic compounds (caffeic acid, catechin, catechol, chlorogenic acid, 3,4-dihydroxyphenylacetic acid, hydroquinone, 4-methylcatechol and vanillin) of nutritional and pharmacological interest. Analytes were detected with UV absorption at λmax 280 and 320 nm. Although catechin and 3-4,dyhidroxyphenylacetic acid showed peak distortion when analyzed by MECC, this technique showed a faster separation ability when compared to RP-HPLC. Moreover, MECC proved to be a powerful tool for the repeatable and reliable quantification of phenolic compounds providing better linearity and area calculation, while RP-HPLC provided the better results in terms of elution time repeatability. Finally, concentration sensitivity in MECC (101-432 μM) were 103 times higher than that of RP-HPLC (90-532 nM). However, both methods had similar minimum detectable mass sensitivity (range: 0.31-2.15 ng) for the selected phenolic compounds.

Analysis of selected phenolic compounds by MECC and HPLC. A comparative study

Versari A.
Membro del Collaboration Group
;
Parpinello G. P.
Membro del Collaboration Group
;
Galassi S.
Membro del Collaboration Group
1999

Abstract

Two methods of analysis, micellar electrokinetic capillary chromatography (MECC) and reversed-phase high-performance liquid chromatography (RP-HPLC), were developed for the separation and determination of selected phenolic compounds (caffeic acid, catechin, catechol, chlorogenic acid, 3,4-dihydroxyphenylacetic acid, hydroquinone, 4-methylcatechol and vanillin) of nutritional and pharmacological interest. Analytes were detected with UV absorption at λmax 280 and 320 nm. Although catechin and 3-4,dyhidroxyphenylacetic acid showed peak distortion when analyzed by MECC, this technique showed a faster separation ability when compared to RP-HPLC. Moreover, MECC proved to be a powerful tool for the repeatable and reliable quantification of phenolic compounds providing better linearity and area calculation, while RP-HPLC provided the better results in terms of elution time repeatability. Finally, concentration sensitivity in MECC (101-432 μM) were 103 times higher than that of RP-HPLC (90-532 nM). However, both methods had similar minimum detectable mass sensitivity (range: 0.31-2.15 ng) for the selected phenolic compounds.
Versari A.; Parpinello G.P.; Galassi S.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/895430
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