We recently described the genetic antimicrobial resistance and virulence profile of a collection of 279 commensal E. coli of food-producing animal (FPA), pet, wildlife and human origin. Phenotypic antimicrobial resistance (AMR) and the role of commensal E. coli as reservoir of extra- intestinal pathogenic Escherichia coli (ExPEC) virulence-associated genes (VAGs) or as potential ExPEC pathogens were evaluated. The most common phenotypic resistance was to tetracycline (76/279, 27.24%), sulfamethoxazole/trimethoprim (73/279, 26.16%), streptomycin and sulfisoxazole (71/279, 25.45% both) among the overall collection. Poultry and rabbit were the sources mostly associated to AMR, with a significant resistance rate (p > 0.01) to quinolones, streptomycin, sulphonamides, tetracycline and, only for poultry, to ampicillin and chloramphenicol. Finally, rabbit was the source mostly associated to colistin resistance. Different pandemic (ST69/69*, ST95, ST131) and emerging (ST10/ST10*, ST23, ST58, ST117, ST405, ST648) ExPEC sequence types (STs) were identified among the collection, especially in poultry source. Both ST groups carried high number of ExPEC VAGs (pandemic ExPEC STs, mean = 8.92; emerging ExPEC STs, mean = 6.43) and showed phenotypic resistance to different antimicrobials (pandemic ExPEC STs, mean = 2.23; emerging ExPEC STs, mean = 2.43), suggesting their role as potential ExPEC pathogens. Variable phenotypic resistance and ExPEC VAG distribution was also observed in uncommon ExPEC lineages, suggesting commensal flora as a potential reservoir of virulence (mean = 3.80) and antimicrobial resistance (mean = 1.69) determinants.

Antimicrobial Resistance Profile and ExPEC Virulence Potential in Commensal Escherichia coli of Multiple Sources / Massella, Elisa; Giacometti, Federica; Bonilauri, Paolo; Reid, Cameron J.; Djordjevic, Steven P.; Merialdi, Giuseppe; Bacci, Cristina; Fiorentini, Laura; Massi, Paola; Bardasi, Lia; Rubini, Silva; Savini, Federica; Serraino, Andrea; Piva, Silvia. - In: ANTIBIOTICS. - ISSN 2079-6382. - STAMPA. - 10:4(2021), pp. 351.1-351.28. [10.3390/antibiotics10040351]

Antimicrobial Resistance Profile and ExPEC Virulence Potential in Commensal Escherichia coli of Multiple Sources

Massella, Elisa
Primo
;
Giacometti, Federica
Secondo
;
Bacci, Cristina;Massi, Paola;Savini, Federica;Serraino, Andrea;Piva, Silvia
Ultimo
2021

Abstract

We recently described the genetic antimicrobial resistance and virulence profile of a collection of 279 commensal E. coli of food-producing animal (FPA), pet, wildlife and human origin. Phenotypic antimicrobial resistance (AMR) and the role of commensal E. coli as reservoir of extra- intestinal pathogenic Escherichia coli (ExPEC) virulence-associated genes (VAGs) or as potential ExPEC pathogens were evaluated. The most common phenotypic resistance was to tetracycline (76/279, 27.24%), sulfamethoxazole/trimethoprim (73/279, 26.16%), streptomycin and sulfisoxazole (71/279, 25.45% both) among the overall collection. Poultry and rabbit were the sources mostly associated to AMR, with a significant resistance rate (p > 0.01) to quinolones, streptomycin, sulphonamides, tetracycline and, only for poultry, to ampicillin and chloramphenicol. Finally, rabbit was the source mostly associated to colistin resistance. Different pandemic (ST69/69*, ST95, ST131) and emerging (ST10/ST10*, ST23, ST58, ST117, ST405, ST648) ExPEC sequence types (STs) were identified among the collection, especially in poultry source. Both ST groups carried high number of ExPEC VAGs (pandemic ExPEC STs, mean = 8.92; emerging ExPEC STs, mean = 6.43) and showed phenotypic resistance to different antimicrobials (pandemic ExPEC STs, mean = 2.23; emerging ExPEC STs, mean = 2.43), suggesting their role as potential ExPEC pathogens. Variable phenotypic resistance and ExPEC VAG distribution was also observed in uncommon ExPEC lineages, suggesting commensal flora as a potential reservoir of virulence (mean = 3.80) and antimicrobial resistance (mean = 1.69) determinants.
2021
Antimicrobial Resistance Profile and ExPEC Virulence Potential in Commensal Escherichia coli of Multiple Sources / Massella, Elisa; Giacometti, Federica; Bonilauri, Paolo; Reid, Cameron J.; Djordjevic, Steven P.; Merialdi, Giuseppe; Bacci, Cristina; Fiorentini, Laura; Massi, Paola; Bardasi, Lia; Rubini, Silva; Savini, Federica; Serraino, Andrea; Piva, Silvia. - In: ANTIBIOTICS. - ISSN 2079-6382. - STAMPA. - 10:4(2021), pp. 351.1-351.28. [10.3390/antibiotics10040351]
Massella, Elisa; Giacometti, Federica; Bonilauri, Paolo; Reid, Cameron J.; Djordjevic, Steven P.; Merialdi, Giuseppe; Bacci, Cristina; Fiorentini, Laura; Massi, Paola; Bardasi, Lia; Rubini, Silva; Savini, Federica; Serraino, Andrea; Piva, Silvia
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/850074
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