A Fast gas chromatography-mass spectrometry method for plant sterols/stanols analysis was developed, using a short capillary gas chromatography column (10 m x 0.1 mm internal diameter x 0.1 μm film thickness) coated with 5% diphenyl-polysiloxane. A silylated mixture of the main plant sterols/stanols standards (beta-sitosterol, campesterol, stigmasterol, campestanol, sitostanol) was well separated in 1.5 min, with a good peak resolution (>1.4, determined on a critical chromatographic peak pair (beta-sitosterol and sitostanol)), repeatability (<13%) and sensitivity (<0.017 ng mL-1). The suitability of this Fast chromatography method was tested on plant sterols/stanols-enriched dairy products (yogurt and milk), which were subjected to lipid extraction, cold saponification and silylation prior to injection. The analytical performance (sensitivity <0.256 ng mL-1 and repeatability <10.36%) and significant reduction of the analysis time and consumables, demonstrates that Fast gas chromatography-mass spectrometry could be also employed for the plant sterols/stanols analysis in functional dairy products.
Analysis of phytosterols and phytostanols in enriched dairy products by Fast gas chromatography-mass spectrometry
INCHINGOLO, RAFFAELLA;CARDENIA, VLADIMIRO;RODRIGUEZ ESTRADA, MARIA TERESA
2014
Abstract
A Fast gas chromatography-mass spectrometry method for plant sterols/stanols analysis was developed, using a short capillary gas chromatography column (10 m x 0.1 mm internal diameter x 0.1 μm film thickness) coated with 5% diphenyl-polysiloxane. A silylated mixture of the main plant sterols/stanols standards (beta-sitosterol, campesterol, stigmasterol, campestanol, sitostanol) was well separated in 1.5 min, with a good peak resolution (>1.4, determined on a critical chromatographic peak pair (beta-sitosterol and sitostanol)), repeatability (<13%) and sensitivity (<0.017 ng mL-1). The suitability of this Fast chromatography method was tested on plant sterols/stanols-enriched dairy products (yogurt and milk), which were subjected to lipid extraction, cold saponification and silylation prior to injection. The analytical performance (sensitivity <0.256 ng mL-1 and repeatability <10.36%) and significant reduction of the analysis time and consumables, demonstrates that Fast gas chromatography-mass spectrometry could be also employed for the plant sterols/stanols analysis in functional dairy products.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
