A Fast gas chromatography-mass spectrometry method for plant sterols/stanols analysis was developed, using a short capillary gas chromatography column (10 m x 0.1 mm internal diameter x 0.1 μm film thickness) coated with 5% diphenyl-polysiloxane. A silylated mixture of the main plant sterols/stanols standards (beta-sitosterol, campesterol, stigmasterol, campestanol, sitostanol) was well separated in 1.5 min, with a good peak resolution (>1.4, determined on a critical chromatographic peak pair (beta-sitosterol and sitostanol)), repeatability (<13%) and sensitivity (<0.017 ng mL-1). The suitability of this Fast chromatography method was tested on plant sterols/stanols-enriched dairy products (yogurt and milk), which were subjected to lipid extraction, cold saponification and silylation prior to injection. The analytical performance (sensitivity <0.256 ng mL-1 and repeatability <10.36%) and significant reduction of the analysis time and consumables, demonstrates that Fast gas chromatography-mass spectrometry could be also employed for the plant sterols/stanols analysis in functional dairy products.

Analysis of phytosterols and phytostanols in enriched dairy products by Fast gas chromatography-mass spectrometry / R. Inchingolo; V. Cardenia; M. T. Rodriguez-Estrada. - In: JOURNAL OF SEPARATION SCIENCE. - ISSN 1615-9314. - ELETTRONICO. - 37:(2014), pp. 2911-2919. [10.1002/jssc.201400322]

Analysis of phytosterols and phytostanols in enriched dairy products by Fast gas chromatography-mass spectrometry

INCHINGOLO, RAFFAELLA;CARDENIA, VLADIMIRO;RODRIGUEZ ESTRADA, MARIA TERESA
2014

Abstract

A Fast gas chromatography-mass spectrometry method for plant sterols/stanols analysis was developed, using a short capillary gas chromatography column (10 m x 0.1 mm internal diameter x 0.1 μm film thickness) coated with 5% diphenyl-polysiloxane. A silylated mixture of the main plant sterols/stanols standards (beta-sitosterol, campesterol, stigmasterol, campestanol, sitostanol) was well separated in 1.5 min, with a good peak resolution (>1.4, determined on a critical chromatographic peak pair (beta-sitosterol and sitostanol)), repeatability (<13%) and sensitivity (<0.017 ng mL-1). The suitability of this Fast chromatography method was tested on plant sterols/stanols-enriched dairy products (yogurt and milk), which were subjected to lipid extraction, cold saponification and silylation prior to injection. The analytical performance (sensitivity <0.256 ng mL-1 and repeatability <10.36%) and significant reduction of the analysis time and consumables, demonstrates that Fast gas chromatography-mass spectrometry could be also employed for the plant sterols/stanols analysis in functional dairy products.
2014
Analysis of phytosterols and phytostanols in enriched dairy products by Fast gas chromatography-mass spectrometry / R. Inchingolo; V. Cardenia; M. T. Rodriguez-Estrada. - In: JOURNAL OF SEPARATION SCIENCE. - ISSN 1615-9314. - ELETTRONICO. - 37:(2014), pp. 2911-2919. [10.1002/jssc.201400322]
R. Inchingolo; V. Cardenia; M. T. Rodriguez-Estrada
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/314320
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