The aim of the present study was to set-up a Fast gas chromatography/mass spectrometry method for the analysis of cholesterol oxidation products (COPs). A silylated mixture of seven oxysterol standards was injected into a Fast GC/MS system. A capillary GC column (10 m × 0.1 mm internal diameter × 0.1 μm film thickness) coated with 95% dimethyl- and 5% diphenyl-polysiloxane, was used. The method gave a fast (total analysis time= 3.5 min) and satisfactory resolution (R > 1.2) of the COPs standards, with a good repeatability and sensitivity, similar to those of conventional GC/MS; recoveries were tested on mice liver. Fast GC/MS method suitability for COPs analysis in food was also tested on an oxidized sardine fillet, which had been previously saponified and purified by NH2 solid phase extraction (SPE); a good repeatability and sensitivity was also obtained. The analytical performance of Fast GC/MS method for the determination of COPs, together with the consequent significant reduction of the analysis time and consumables, demonstrates that Fast GC/MS represents a valid alternative to conventional GC/MS and evinces the great potential of such analytical technique, which could be applied to both food and biological samples.

Analysis of cholesterol oxidation products by Fast gas chromatography/mass spectrometry

CARDENIA, VLADIMIRO;RODRIGUEZ ESTRADA, MARIA TERESA;SAVIOLI, STEFANO;LERCKER, GIOVANNI
2012

Abstract

The aim of the present study was to set-up a Fast gas chromatography/mass spectrometry method for the analysis of cholesterol oxidation products (COPs). A silylated mixture of seven oxysterol standards was injected into a Fast GC/MS system. A capillary GC column (10 m × 0.1 mm internal diameter × 0.1 μm film thickness) coated with 95% dimethyl- and 5% diphenyl-polysiloxane, was used. The method gave a fast (total analysis time= 3.5 min) and satisfactory resolution (R > 1.2) of the COPs standards, with a good repeatability and sensitivity, similar to those of conventional GC/MS; recoveries were tested on mice liver. Fast GC/MS method suitability for COPs analysis in food was also tested on an oxidized sardine fillet, which had been previously saponified and purified by NH2 solid phase extraction (SPE); a good repeatability and sensitivity was also obtained. The analytical performance of Fast GC/MS method for the determination of COPs, together with the consequent significant reduction of the analysis time and consumables, demonstrates that Fast GC/MS represents a valid alternative to conventional GC/MS and evinces the great potential of such analytical technique, which could be applied to both food and biological samples.
2012
V. Cardenia; M. T. Rodriguez-Estrada; E. Baldacci; S. Savioli; G. Lercker
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/106430
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