Dietary fiber has been shown to have multiple health benefits, including a positive effect on longevity and the gut microbiota. In the present study, Drosophila melanogaster has been chosen as an in vivo model organism to study the health effects of dietary fiber supplementation (DFS). DFS extended the mean half-life of male and female flies, but the absolute lifespan only increased in females. To reveal the underlying mechanisms, we examined the effect of DFS on gut microbiota diversity and abundance, local gut immunity, and the brain proteome. A significant difference in the gut microbial community was observed between groups with and without fiber supplementation, which reduced the gut pathogenic bacterial load. We also observed an upregulated expression of dual oxidase and a modulated expression of Attacin and Diptericin genes in the gut of older flies, possibly delaying the gut dysbiosis connected to the age-related gut immune dysfunction. Brain proteome analysis showed that DFS led to the modulation of metabolic processes connected to mitochondrial biogenesis, the RhoV-GTPase cycle, organelle biogenesis and maintenance, membrane trafficking and vesicle-mediated transport, possibly orchestrated through a gut–brain axis interaction. Taken together, our study shows that DFS can prolong the half-life and lifespan of flies, possibly by promoting a healthier gut environment and delaying the physiological dysbiosis that characterizes the ageing process. However, the RhoV-GTPase cycle at the brain level may deserve more attention in future studies.
Beghelli D, G.L. (2024). Dietary fiber supplementation increases Drosophila melanogaster lifespan and gut microbiota diversity. FOOD & FUNCTION, 15(14), 7468-7477 [10.1039/d4fo00879k].
Dietary fiber supplementation increases Drosophila melanogaster lifespan and gut microbiota diversity.
Hrelia SWriting – Review & Editing
;Angeloni C.Ultimo
Conceptualization
2024
Abstract
Dietary fiber has been shown to have multiple health benefits, including a positive effect on longevity and the gut microbiota. In the present study, Drosophila melanogaster has been chosen as an in vivo model organism to study the health effects of dietary fiber supplementation (DFS). DFS extended the mean half-life of male and female flies, but the absolute lifespan only increased in females. To reveal the underlying mechanisms, we examined the effect of DFS on gut microbiota diversity and abundance, local gut immunity, and the brain proteome. A significant difference in the gut microbial community was observed between groups with and without fiber supplementation, which reduced the gut pathogenic bacterial load. We also observed an upregulated expression of dual oxidase and a modulated expression of Attacin and Diptericin genes in the gut of older flies, possibly delaying the gut dysbiosis connected to the age-related gut immune dysfunction. Brain proteome analysis showed that DFS led to the modulation of metabolic processes connected to mitochondrial biogenesis, the RhoV-GTPase cycle, organelle biogenesis and maintenance, membrane trafficking and vesicle-mediated transport, possibly orchestrated through a gut–brain axis interaction. Taken together, our study shows that DFS can prolong the half-life and lifespan of flies, possibly by promoting a healthier gut environment and delaying the physiological dysbiosis that characterizes the ageing process. However, the RhoV-GTPase cycle at the brain level may deserve more attention in future studies.File | Dimensione | Formato | |
---|---|---|---|
Food and Funtion final.pdf
accesso aperto
Descrizione: beghelli et al. published
Tipo:
Versione (PDF) editoriale
Licenza:
Licenza per Accesso Aperto. Creative Commons Attribuzione (CCBY)
Dimensione
696.04 kB
Formato
Adobe PDF
|
696.04 kB | Adobe PDF | Visualizza/Apri |
Supplementary 1.pdf
accesso aperto
Descrizione: supplementary material 1
Tipo:
File Supplementare
Licenza:
Licenza per Accesso Aperto. Creative Commons Attribuzione (CCBY)
Dimensione
183.47 kB
Formato
Adobe PDF
|
183.47 kB | Adobe PDF | Visualizza/Apri |
Supplementary 2.pdf
accesso aperto
Descrizione: supplementary material 2
Tipo:
File Supplementare
Licenza:
Licenza per Accesso Aperto. Creative Commons Attribuzione (CCBY)
Dimensione
266.53 kB
Formato
Adobe PDF
|
266.53 kB | Adobe PDF | Visualizza/Apri |
Supplementary 3.pdf
accesso aperto
Descrizione: supplementary material 3
Tipo:
File Supplementare
Licenza:
Licenza per Accesso Aperto. Creative Commons Attribuzione (CCBY)
Dimensione
267.24 kB
Formato
Adobe PDF
|
267.24 kB | Adobe PDF | Visualizza/Apri |
Supplementary 4.pdf
accesso aperto
Descrizione: supplementary material 4
Tipo:
File Supplementare
Licenza:
Licenza per Accesso Aperto. Creative Commons Attribuzione (CCBY)
Dimensione
157.06 kB
Formato
Adobe PDF
|
157.06 kB | Adobe PDF | Visualizza/Apri |
Supplementary 5.pdf
accesso aperto
Descrizione: supplementary material 5
Tipo:
File Supplementare
Licenza:
Licenza per Accesso Aperto. Creative Commons Attribuzione (CCBY)
Dimensione
135.14 kB
Formato
Adobe PDF
|
135.14 kB | Adobe PDF | Visualizza/Apri |
Supplementary 6.pdf
accesso aperto
Descrizione: supplementary material 6
Tipo:
File Supplementare
Licenza:
Licenza per Accesso Aperto. Creative Commons Attribuzione (CCBY)
Dimensione
173.51 kB
Formato
Adobe PDF
|
173.51 kB | Adobe PDF | Visualizza/Apri |
Supplementary 7.pdf
accesso aperto
Descrizione: supplementary material 7
Tipo:
File Supplementare
Licenza:
Licenza per Accesso Aperto. Creative Commons Attribuzione (CCBY)
Dimensione
148.33 kB
Formato
Adobe PDF
|
148.33 kB | Adobe PDF | Visualizza/Apri |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.