In human airway epithelial cell lines 9HTEo- and CFNPE9o-, histamine causes a transient elevation of intracellular free calcium concentration ([Ca2+](i)) detected by fura 2 fluorescence, which is due to both release from intracellular stores and extracellular Ca2+ entry. The effect of histamine is abolished by the Ca2+-ATPase inhibitor thapsigargin. Histamine also stimulates inositol phosphate accumulation. Changes in [Ca2+](i) and inositol phosphate production exhibit a similar dose- response relationship for histamine (maximal effect at 10-4 M), with both phenomena being blocked by the H1 antagonist mepyramine and being insensitive to pertussis toxin treatment. The effects of histamine on phosphoinositide metabolism and [Ca2+](i) are abolished by a short-term preincubation with phorbol ester, and this effect is reversed by staurosporine and calphostin C, suggesting a feedback regulation by protein kinase C. The results indicate that human airway epithelial cells contain H1 receptors coupled to phospholipase C through a pertussis toxin- insensitive G protein.
Histamine activates phospholipase C in human airway epithelial cells via a phorbol ester-sensitive pathway / Rugolo M.; Barzanti F.; Gruenert D.C.; Hrelia S.. - In: AMERICAN JOURNAL OF PHYSIOLOGY. LUNG CELLULAR AND MOLECULAR PHYSIOLOGY. - ISSN 1040-0605. - STAMPA. - 271:4(1996), pp. L665-L671. [10.1152/ajplung.1996.271.4.l665]
Histamine activates phospholipase C in human airway epithelial cells via a phorbol ester-sensitive pathway
Rugolo M.
Primo
Writing – Original Draft Preparation
;Hrelia S.Ultimo
Writing – Original Draft Preparation
1996
Abstract
In human airway epithelial cell lines 9HTEo- and CFNPE9o-, histamine causes a transient elevation of intracellular free calcium concentration ([Ca2+](i)) detected by fura 2 fluorescence, which is due to both release from intracellular stores and extracellular Ca2+ entry. The effect of histamine is abolished by the Ca2+-ATPase inhibitor thapsigargin. Histamine also stimulates inositol phosphate accumulation. Changes in [Ca2+](i) and inositol phosphate production exhibit a similar dose- response relationship for histamine (maximal effect at 10-4 M), with both phenomena being blocked by the H1 antagonist mepyramine and being insensitive to pertussis toxin treatment. The effects of histamine on phosphoinositide metabolism and [Ca2+](i) are abolished by a short-term preincubation with phorbol ester, and this effect is reversed by staurosporine and calphostin C, suggesting a feedback regulation by protein kinase C. The results indicate that human airway epithelial cells contain H1 receptors coupled to phospholipase C through a pertussis toxin- insensitive G protein.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.