BRCA2 and RAD51 are two key proteins involved in the homologous recombination. BRCA2 interacts with RAD51 through eight repetitive sequences known as BRC repeats. While the interaction taking place among the fourth BRC repeat and RAD51 has already been structurally elucidated, it is still unclear how the other BRC repeats interact with RAD51. The purpose of this project is to gain novel structural details on this interaction by co-expressing and co-purifying BRCA2 truncates, containing multiple BRC repeats, and RAD51.
Unveiling the molecular mechanism of BRCA2-RAD51 interaction to tackle cancer onset / Rinaldi F., Schipani F., Scietti L., Girotto S, Cavalli A.. - STAMPA. - (2022). (Intervento presentato al convegno EMBO Practical Course "Characterization of macromolecular complexes by integrative structural biology" tenutosi a Grenoble nel 27/05/2022).
Unveiling the molecular mechanism of BRCA2-RAD51 interaction to tackle cancer onset
Rinaldi F.;Cavalli A.
2022
Abstract
BRCA2 and RAD51 are two key proteins involved in the homologous recombination. BRCA2 interacts with RAD51 through eight repetitive sequences known as BRC repeats. While the interaction taking place among the fourth BRC repeat and RAD51 has already been structurally elucidated, it is still unclear how the other BRC repeats interact with RAD51. The purpose of this project is to gain novel structural details on this interaction by co-expressing and co-purifying BRCA2 truncates, containing multiple BRC repeats, and RAD51.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
