The earlieststudiesconcerningpolyamines(PAs)inplantswereperformedbyusing in vitro cultured explantsof Helianthustuberosus dormanttuber.Thisparenchymatissuewasparticularlyusefulduetoits susceptibility toseveralgrowthsubstances,includingPAs.Duringtuberdormancy,PAlevelsaretoolowto sustaincelldivision;thus Helianthus representsanaturalPA-deficient model.Whencultivated in vitro in the presenceofauxins, Helianthus tuber dormantparenchymacellsattheG0 stage starttodivide synchronously acquiringmeristematiccharacteristics.Therequirementforauxinstoinducecelldivision can besubstitutedbyaliphaticPAssuchasputrescine,spermidineorspermine.Cylindersorslicesof explantedhomogeneoustuberparenchymawereculturedinliquidmediumforshort-termstudiesonthe cell cycle,oronsolidagarmediumforlong-termexperiments.Morphologicalandphysiologicalmodificationsofsynchronouslydividingcellswerestudiedduringthedifferentphasesofthecellcycleinrelat on to PAsbiosynthesisandoxidation.Long-termexperimentsledtotheidentification ofthePAsasplant growthregulators,asthesolenitrogensource,astuberstoragesubstancesandasessentialfactorsfor morphogeneticprocessesandcellhomeostasis.Morerecentlythissystemwasusedtostudytheeffectson plant cellproliferationofplatinum-orpalladium-deriveddrugs(cisplatinandplatinumorpalladiumbi- substitutedspermine)thatareusedinhumancancercelllinesasantiproliferativeandcytotoxicagents. CisplatinwasthemostactivebothincellproliferationinhibitionandonPAmetabolism.Similarexperi- ments wereperformedusingthreeagmatineanalogous.Differenteffectsofthesecompoundswere observedoncellproliferation,freePAlevelsandenzymeactivities,leadingtoahypothesisofacorrelation between theirchemicalstructureandtheagmatinemetabolisminplants
Tassoni A., Bagni N., Ferri M., Franceschetti M., Khomutov A., Marques M.P., et al. (2010). Helianthus tuberosus and polyamine research: past and recent applications of a classical growth model. PLANT PHYSIOLOGY AND BIOCHEMISTRY, 48, 496-505 [10.1016/j.plaphy.2010.01.019].
Helianthus tuberosus and polyamine research: past and recent applications of a classical growth model
TASSONI, ANNALISA;BAGNI, NELLO;FERRI, MAURA;FRANCESCHETTI, MARINA;SERAFINI FRACASSINI, DONATELLA
2010
Abstract
The earlieststudiesconcerningpolyamines(PAs)inplantswereperformedbyusing in vitro cultured explantsof Helianthustuberosus dormanttuber.Thisparenchymatissuewasparticularlyusefulduetoits susceptibility toseveralgrowthsubstances,includingPAs.Duringtuberdormancy,PAlevelsaretoolowto sustaincelldivision;thus Helianthus representsanaturalPA-deficient model.Whencultivated in vitro in the presenceofauxins, Helianthus tuber dormantparenchymacellsattheG0 stage starttodivide synchronously acquiringmeristematiccharacteristics.Therequirementforauxinstoinducecelldivision can besubstitutedbyaliphaticPAssuchasputrescine,spermidineorspermine.Cylindersorslicesof explantedhomogeneoustuberparenchymawereculturedinliquidmediumforshort-termstudiesonthe cell cycle,oronsolidagarmediumforlong-termexperiments.Morphologicalandphysiologicalmodificationsofsynchronouslydividingcellswerestudiedduringthedifferentphasesofthecellcycleinrelat on to PAsbiosynthesisandoxidation.Long-termexperimentsledtotheidentification ofthePAsasplant growthregulators,asthesolenitrogensource,astuberstoragesubstancesandasessentialfactorsfor morphogeneticprocessesandcellhomeostasis.Morerecentlythissystemwasusedtostudytheeffectson plant cellproliferationofplatinum-orpalladium-deriveddrugs(cisplatinandplatinumorpalladiumbi- substitutedspermine)thatareusedinhumancancercelllinesasantiproliferativeandcytotoxicagents. CisplatinwasthemostactivebothincellproliferationinhibitionandonPAmetabolism.Similarexperi- ments wereperformedusingthreeagmatineanalogous.Differenteffectsofthesecompoundswere observedoncellproliferation,freePAlevelsandenzymeactivities,leadingtoahypothesisofacorrelation between theirchemicalstructureandtheagmatinemetabolisminplantsI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
