The earlieststudiesconcerningpolyamines(PAs)inplantswereperformedbyusing in vitro cultured explantsof Helianthustuberosus dormanttuber.Thisparenchymatissuewasparticularlyusefulduetoits susceptibility toseveralgrowthsubstances,includingPAs.Duringtuberdormancy,PAlevelsaretoolowto sustaincelldivision;thus Helianthus representsanaturalPA-deficient model.Whencultivated in vitro in the presenceofauxins, Helianthus tuber dormantparenchymacellsattheG0 stage starttodivide synchronously acquiringmeristematiccharacteristics.Therequirementforauxinstoinducecelldivision can besubstitutedbyaliphaticPAssuchasputrescine,spermidineorspermine.Cylindersorslicesof explantedhomogeneoustuberparenchymawereculturedinliquidmediumforshort-termstudiesonthe cell cycle,oronsolidagarmediumforlong-termexperiments.Morphologicalandphysiologicalmodificationsofsynchronouslydividingcellswerestudiedduringthedifferentphasesofthecellcycleinrelat on to PAsbiosynthesisandoxidation.Long-termexperimentsledtotheidentification ofthePAsasplant growthregulators,asthesolenitrogensource,astuberstoragesubstancesandasessentialfactorsfor morphogeneticprocessesandcellhomeostasis.Morerecentlythissystemwasusedtostudytheeffectson plant cellproliferationofplatinum-orpalladium-deriveddrugs(cisplatinandplatinumorpalladiumbi- substitutedspermine)thatareusedinhumancancercelllinesasantiproliferativeandcytotoxicagents. CisplatinwasthemostactivebothincellproliferationinhibitionandonPAmetabolism.Similarexperi- ments wereperformedusingthreeagmatineanalogous.Differenteffectsofthesecompoundswere observedoncellproliferation,freePAlevelsandenzymeactivities,leadingtoahypothesisofacorrelation between theirchemicalstructureandtheagmatinemetabolisminplants
Helianthus tuberosus and polyamine research: past and recent applications of a classical growth model / Tassoni A.; Bagni N.; Ferri M.; Franceschetti M.; Khomutov A.; Marques M.P.; Fiuza S.M.; Simonian A.R.; Serafini-Fracassini D.. - In: PLANT PHYSIOLOGY AND BIOCHEMISTRY. - ISSN 0981-9428. - STAMPA. - 48:(2010), pp. 496-505. [10.1016/j.plaphy.2010.01.019]
Helianthus tuberosus and polyamine research: past and recent applications of a classical growth model
TASSONI, ANNALISA;BAGNI, NELLO;FERRI, MAURA;FRANCESCHETTI, MARINA;SERAFINI FRACASSINI, DONATELLA
2010
Abstract
The earlieststudiesconcerningpolyamines(PAs)inplantswereperformedbyusing in vitro cultured explantsof Helianthustuberosus dormanttuber.Thisparenchymatissuewasparticularlyusefulduetoits susceptibility toseveralgrowthsubstances,includingPAs.Duringtuberdormancy,PAlevelsaretoolowto sustaincelldivision;thus Helianthus representsanaturalPA-deficient model.Whencultivated in vitro in the presenceofauxins, Helianthus tuber dormantparenchymacellsattheG0 stage starttodivide synchronously acquiringmeristematiccharacteristics.Therequirementforauxinstoinducecelldivision can besubstitutedbyaliphaticPAssuchasputrescine,spermidineorspermine.Cylindersorslicesof explantedhomogeneoustuberparenchymawereculturedinliquidmediumforshort-termstudiesonthe cell cycle,oronsolidagarmediumforlong-termexperiments.Morphologicalandphysiologicalmodificationsofsynchronouslydividingcellswerestudiedduringthedifferentphasesofthecellcycleinrelat on to PAsbiosynthesisandoxidation.Long-termexperimentsledtotheidentification ofthePAsasplant growthregulators,asthesolenitrogensource,astuberstoragesubstancesandasessentialfactorsfor morphogeneticprocessesandcellhomeostasis.Morerecentlythissystemwasusedtostudytheeffectson plant cellproliferationofplatinum-orpalladium-deriveddrugs(cisplatinandplatinumorpalladiumbi- substitutedspermine)thatareusedinhumancancercelllinesasantiproliferativeandcytotoxicagents. CisplatinwasthemostactivebothincellproliferationinhibitionandonPAmetabolism.Similarexperi- ments wereperformedusingthreeagmatineanalogous.Differenteffectsofthesecompoundswere observedoncellproliferation,freePAlevelsandenzymeactivities,leadingtoahypothesisofacorrelation between theirchemicalstructureandtheagmatinemetabolisminplantsI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
