This study describes the characterization of phenolic compounds in buckwheat. Reverse phase high performance liquid chromatography (RP-HPLC) coupled to electrospray ionization-time of flight-mass spectrometry (ESI-TOF-MS) has been applied to separate and characterize thirty phenolic compounds in buckwheat flour. As far as we know, 2-hydroxy-3-O-β-d-glucopyranosil-benzoic acid, 1-O-caffeoyl-6-O-alpha-rhamnopyranosyl-β-glycopyranoside and epicatechin-3-(3″-O-methyl) gallate were tentatively identified in buckwheat for the first time. The sensitivity, mass accuracy and true isotopic pattern of the TOF-MS, legitimated the identification of phenolic compounds present in buckwheat extract. Furthermore, other “unknown” compounds were also reported in the ethanolic extracts of buckwheat.

Verardo V. , Arráez-Román D. , Segura-Carretero A. , Marconi E. , Fernández-Gutiérrez A., Caboni M.F. (2010). Identification of buckwheat phenolic compounds by reverse phase high performance liquid chromatography–electrospray ionization-time of flight-mass spectrometry (RP-HPLC – ESI-TOF-MS). JOURNAL OF CEREAL SCIENCE, 52, 170-176 [10.1016/j.jcs.2010.04.009].

Identification of buckwheat phenolic compounds by reverse phase high performance liquid chromatography–electrospray ionization-time of flight-mass spectrometry (RP-HPLC – ESI-TOF-MS).

VERARDO, VITO;CABONI, MARIA
2010

Abstract

This study describes the characterization of phenolic compounds in buckwheat. Reverse phase high performance liquid chromatography (RP-HPLC) coupled to electrospray ionization-time of flight-mass spectrometry (ESI-TOF-MS) has been applied to separate and characterize thirty phenolic compounds in buckwheat flour. As far as we know, 2-hydroxy-3-O-β-d-glucopyranosil-benzoic acid, 1-O-caffeoyl-6-O-alpha-rhamnopyranosyl-β-glycopyranoside and epicatechin-3-(3″-O-methyl) gallate were tentatively identified in buckwheat for the first time. The sensitivity, mass accuracy and true isotopic pattern of the TOF-MS, legitimated the identification of phenolic compounds present in buckwheat extract. Furthermore, other “unknown” compounds were also reported in the ethanolic extracts of buckwheat.
2010
Verardo V. , Arráez-Román D. , Segura-Carretero A. , Marconi E. , Fernández-Gutiérrez A., Caboni M.F. (2010). Identification of buckwheat phenolic compounds by reverse phase high performance liquid chromatography–electrospray ionization-time of flight-mass spectrometry (RP-HPLC – ESI-TOF-MS). JOURNAL OF CEREAL SCIENCE, 52, 170-176 [10.1016/j.jcs.2010.04.009].
Verardo V. ; Arráez-Román D. ; Segura-Carretero A. ; Marconi E. ; Fernández-Gutiérrez A.; Caboni M.F.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/90887
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