Earthworm coelomocytes have been used as effector cells against the human tumor target, K562. To first assess the viability of effectors, incorporation of [3H]thymidine was tested and was higher in autogeneic (A ⇔ A, self) than in allogeneic (A ⇔ B, nonself) coelomocytes. A ⇔ A showed significantly greater numbers in S, G2, or M phases than A ⇔ B coelomocytes. When A ⇔ A or A ⇔ B were cultured, no significant cell killing occurred in either, as measured in a 4-hr51Cr release assay. A ⇔ A but not A ⇔ B killed K562 target cells. Cytotoxicity was dependent upon membrane binding between small, electron-dense coelomocytes and targets; it was enhanced by adding PHA. The heat labile supernatant from A ⇔ A but not from A ⇔ B killed K562 targets after cultivation for 10 min at 22°C, but not immediately after washing. Recognition of, binding to, and killing of foreign cells in a natural killer cell-like reaction may reflect natural immunity in earthworms. © 1995 Academic Press. All rights reserved.
Cooper E.L., Cossarizza A., Suzuki M.M., Salvioli S., Capri M., Quaglino D., et al. (1995). Autogeneic but not allogeneic earthworm effector coelomocytes kill the mammalian tumor cell target K562. CELLULAR IMMUNOLOGY, 166(1), 113-122 [10.1006/cimm.1995.0013].
Autogeneic but not allogeneic earthworm effector coelomocytes kill the mammalian tumor cell target K562
Salvioli S.;Capri M.;Franceschi C.
1995
Abstract
Earthworm coelomocytes have been used as effector cells against the human tumor target, K562. To first assess the viability of effectors, incorporation of [3H]thymidine was tested and was higher in autogeneic (A ⇔ A, self) than in allogeneic (A ⇔ B, nonself) coelomocytes. A ⇔ A showed significantly greater numbers in S, G2, or M phases than A ⇔ B coelomocytes. When A ⇔ A or A ⇔ B were cultured, no significant cell killing occurred in either, as measured in a 4-hr51Cr release assay. A ⇔ A but not A ⇔ B killed K562 target cells. Cytotoxicity was dependent upon membrane binding between small, electron-dense coelomocytes and targets; it was enhanced by adding PHA. The heat labile supernatant from A ⇔ A but not from A ⇔ B killed K562 targets after cultivation for 10 min at 22°C, but not immediately after washing. Recognition of, binding to, and killing of foreign cells in a natural killer cell-like reaction may reflect natural immunity in earthworms. © 1995 Academic Press. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
