A reliable high-performance liquid chromatography-refractive index detection method for the simultaneous analysis of glucose, methanol, and glycerol in a bioprocess fermentation media using direct injection is presented. The validation studies show a satisfactory selectivity, linearity, accuracy, and recovery of the method. The lowest concentration detectable for glucose and methanol is 3.5 and 6.7 mg/100 mL, respectively. This method could be an attractive choice for the analysis of these compounds not only in fermentation media but also in biomedical and environmental samples.
Parpinello G.P., Versari A. (2000). A simple high-performance liquid chromatography method for the analysis of glucose, glycerol, and methanol in a bioprocess. JOURNAL OF CHROMATOGRAPHIC SCIENCE, 38(6), 259-261 [10.1093/chromsci/38.6.259].
A simple high-performance liquid chromatography method for the analysis of glucose, glycerol, and methanol in a bioprocess
Parpinello G. P.Membro del Collaboration Group
;Versari A.
Membro del Collaboration Group
2000
Abstract
A reliable high-performance liquid chromatography-refractive index detection method for the simultaneous analysis of glucose, methanol, and glycerol in a bioprocess fermentation media using direct injection is presented. The validation studies show a satisfactory selectivity, linearity, accuracy, and recovery of the method. The lowest concentration detectable for glucose and methanol is 3.5 and 6.7 mg/100 mL, respectively. This method could be an attractive choice for the analysis of these compounds not only in fermentation media but also in biomedical and environmental samples.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
