Aggregation is among the most critical parameters affecting the pharmacological and safety profile of peptide Active Pharmaceutical Ingredients (APIs). For this reason, it is of utmost importance to define the exact aggregation state of peptide drugs, particularly when the API is marketed as a ready-to-use solution. Consequently, appropriate non-destructive techniques able to replicate the peptide environment must be employed. In our work, we exploited Asymmetrical Flow Field-Flow Fractionation (AF4), connected to UV, dRI, fluorescence, and MALS detectors, to fully characterize the aggregation state of Liraglutide, a peptide API used for the treatment of diabetes type 2 and chronic obesity. In previous studies, Liraglutide was hypothesized to assemble into hexa-octamers in phosphate buffer, but no information on its behavior in the formulation medium was provided up to now. The method used allowed researchers to work using formulation as the mobile phase with excellent recoveries and LoQ/LoD, discerning between stable and degraded samples, and detecting, when present, aggregates up to 108 Da. The native state of Liraglutide was assessed and found to be an association into pentamers, with a non-spherical conformation. Combined to benchmark analyses, the sameness study was complete and descriptive, also giving insight on the aggregation process and covalent/non-covalent aggregate types.

Marassi, V., Macis, M., Giordani, S., Ferrazzano, L., Tolomelli, A., Roda, B., et al. (2022). Application of Af4-Multidetection to Liraglutide in Its Formulation: Preserving and Representing Native Aggregation. MOLECULES, 27(17), 1-14 [10.3390/molecules27175485].

Application of Af4-Multidetection to Liraglutide in Its Formulation: Preserving and Representing Native Aggregation

Marassi,V.
Primo
;
Giordani,S.;Ferrazzano,L.;Tolomelli,A.;Roda,B.;Zattoni,A.;Reschiglian,P.;Cabri,W.
Ultimo
Conceptualization
2022

Abstract

Aggregation is among the most critical parameters affecting the pharmacological and safety profile of peptide Active Pharmaceutical Ingredients (APIs). For this reason, it is of utmost importance to define the exact aggregation state of peptide drugs, particularly when the API is marketed as a ready-to-use solution. Consequently, appropriate non-destructive techniques able to replicate the peptide environment must be employed. In our work, we exploited Asymmetrical Flow Field-Flow Fractionation (AF4), connected to UV, dRI, fluorescence, and MALS detectors, to fully characterize the aggregation state of Liraglutide, a peptide API used for the treatment of diabetes type 2 and chronic obesity. In previous studies, Liraglutide was hypothesized to assemble into hexa-octamers in phosphate buffer, but no information on its behavior in the formulation medium was provided up to now. The method used allowed researchers to work using formulation as the mobile phase with excellent recoveries and LoQ/LoD, discerning between stable and degraded samples, and detecting, when present, aggregates up to 108 Da. The native state of Liraglutide was assessed and found to be an association into pentamers, with a non-spherical conformation. Combined to benchmark analyses, the sameness study was complete and descriptive, also giving insight on the aggregation process and covalent/non-covalent aggregate types.
2022
Marassi, V., Macis, M., Giordani, S., Ferrazzano, L., Tolomelli, A., Roda, B., et al. (2022). Application of Af4-Multidetection to Liraglutide in Its Formulation: Preserving and Representing Native Aggregation. MOLECULES, 27(17), 1-14 [10.3390/molecules27175485].
Marassi,V.; Macis,M.; Giordani,S.; Ferrazzano,L.; Tolomelli,A.; Roda,B.; Zattoni,A.; Ricci,A.; Reschiglian,P.; Cabri,W.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/894762
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