Cholesterol oxidation products (COPs) have been associated with the genesis of chronic degenerative diseases, such as atherosclerosis. The purpose of this work was to study the histological changes by toxic effects of dietary COPs in liver and kidney. Five-week old male Wistar albino rats were randomly divided into three groups of ten rats each. Standard rat chow was supplemented with either 1% (w/w) pure cholesterol or 1% oxidized cholesterol and fed to the rats for 8 weeks. Control animals were fed standard rat chow. At the end of the treatment period, the serum lipids profile was determined. The aorta, liver and kidneys were excised immediately, frozen with liquid nitrogen, and held at -70°C. The histological study was carried out using conventional hematoxylin-eosin staining, and histochemical red oil “O” was applied. COPs were analyzed by gas chromatography. Intake of dietary COPs altered biochemical parameters involved in lipid metabolism, which are associated with atherogenesis in rats: total cholesterol, triacylglycerols, and low density lipoproteins in serum. COPs detected in the liver and kidneys, modified the organ original structure, caused an inflammatory process and promoted atherogenesis and athropy of the tissue.
Biochemical and histopathological effects of dietary oxidized cholesterol in rats / I. Soto-Rodríguez; P. J. Campillo-Velázquez; A. Alexander-Aguilera; M. T. Rodriguez-Estrada; G. Lercker; H. S. Garcia. - In: JOURNAL OF APPLIED TOXICOLOGY. - ISSN 0260-437X. - STAMPA. - 29:(2009), pp. 715-723. [10.1002/jat.1463]
Biochemical and histopathological effects of dietary oxidized cholesterol in rats
RODRIGUEZ ESTRADA, MARIA TERESA;LERCKER, GIOVANNI;
2009
Abstract
Cholesterol oxidation products (COPs) have been associated with the genesis of chronic degenerative diseases, such as atherosclerosis. The purpose of this work was to study the histological changes by toxic effects of dietary COPs in liver and kidney. Five-week old male Wistar albino rats were randomly divided into three groups of ten rats each. Standard rat chow was supplemented with either 1% (w/w) pure cholesterol or 1% oxidized cholesterol and fed to the rats for 8 weeks. Control animals were fed standard rat chow. At the end of the treatment period, the serum lipids profile was determined. The aorta, liver and kidneys were excised immediately, frozen with liquid nitrogen, and held at -70°C. The histological study was carried out using conventional hematoxylin-eosin staining, and histochemical red oil “O” was applied. COPs were analyzed by gas chromatography. Intake of dietary COPs altered biochemical parameters involved in lipid metabolism, which are associated with atherogenesis in rats: total cholesterol, triacylglycerols, and low density lipoproteins in serum. COPs detected in the liver and kidneys, modified the organ original structure, caused an inflammatory process and promoted atherogenesis and athropy of the tissue.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
