The main aim of this study was to evaluate the genetic diversity between and within Campylobacter spp. and Clostridium perfringens broiler isolates collected in three different EU countries. A total of 190 Campylobacters (i.e. 63 C. coli and 127 C. jejuni) from the Czech Republic, Germany and Italy and 94 Clostridium perfringens from the Czech Republic and Italy were automatically ribotyped by HaeIII and EcoRI, respectively. Overall, 97 different ribotypes were identified for Campylobacter and 45 for Clostridium perfringens. In particular, 38 Campylobacter ribotypes were shared between isolates and 59 were associated to single strains. Furthermore, 14 Clostridium perfringens profiles were shared between isolates and 31 were associated to single strains. Finally, 6 and 3 Campylobacter and Clostridium perfringens ribotypes, respectively, were associated to strains collected from broilers reared in different countries. This study was supported by project FOOD-CT-200X-007076 (www.poultryflorgut.org)
G. MANFREDA, I. STEINHAUSEROVA, G. BORILOVA, G. KLEIN, M. LANGEN, U. PETERS, et al. (2008). COMPARATIVE ANALYSIS OF THE MOLECULAR PROFILES ASSOCIATED TO CAMPYLOBACTER SPP. AND CLOSTRIDIUM PERFRINGENS BROILER STRAINS COLLECTED IN THE CZECH REPUBLIC, GERMANY AND ITALY. s.l : s.n.
COMPARATIVE ANALYSIS OF THE MOLECULAR PROFILES ASSOCIATED TO CAMPYLOBACTER SPP. AND CLOSTRIDIUM PERFRINGENS BROILER STRAINS COLLECTED IN THE CZECH REPUBLIC, GERMANY AND ITALY
MANFREDA, GERARDO;DE CESARE, ALESSANDRA
2008
Abstract
The main aim of this study was to evaluate the genetic diversity between and within Campylobacter spp. and Clostridium perfringens broiler isolates collected in three different EU countries. A total of 190 Campylobacters (i.e. 63 C. coli and 127 C. jejuni) from the Czech Republic, Germany and Italy and 94 Clostridium perfringens from the Czech Republic and Italy were automatically ribotyped by HaeIII and EcoRI, respectively. Overall, 97 different ribotypes were identified for Campylobacter and 45 for Clostridium perfringens. In particular, 38 Campylobacter ribotypes were shared between isolates and 59 were associated to single strains. Furthermore, 14 Clostridium perfringens profiles were shared between isolates and 31 were associated to single strains. Finally, 6 and 3 Campylobacter and Clostridium perfringens ribotypes, respectively, were associated to strains collected from broilers reared in different countries. This study was supported by project FOOD-CT-200X-007076 (www.poultryflorgut.org)I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
