Chemical profile and antioxidant activity of sage herbal dust extracts obtained by supercritical fluid extraction

The main aims of this research were chemical profiling of essential oil and lipid extracts of sage herbal dust obtained by traditional (hydrodistillation and Soxhlet extraction) and modern extraction techniques (supercritical fluid extraction – SFE), as well as optimization of the SFE process using Artificial Neural Networks (ANNs). Chemical profile of lipid extracts obtained at different set of SFE pressure (100–300 bar), temperature (40–60 °C) and CO2flow rate (0.2–0.4 kg/h) were compared with essential oil and lipid extracts obtained by conventional techniques. The most abundant compounds in all samples were oxygenated monoterpenes (camphor, α-thujone and eucalyptol), sesquiterpenes (viridiflorol) and diterpene polyphenols (epirosmanol). SFE provided significant advantages in terms of monoterpene yield and selectivity comparing to traditional techniques. Antioxidant activity of lipid extracts obtained by SFE was determined by DPPH and FRAP assays. Furthermore, multi-response optimization of SFE process was performed by ANNs, and optimized conditions for maximized total extraction yield and antioxidant activity were pressure of 297.52 bar, temperature of 44.39 °C and CO2flow rate of 0.40 kg/h.