Objective:Immunofluorescence diagnostic systems cost is often dominated by high-sensitivity, low-noise CCD-based cameras which are used to acquire the fluorescence images. In this paper we investigate the use of low-cost CMOS sensors in a point-of-care immunofluorescence diagnostic application for the detection and discrimination of four different serotypes of the Dengue virus in a set of human samples. Methods: A two-phase post-processing software pipeline is proposed which consists in a first image enhancement stage for resolution increasing and segmentation, and a second diagnosis stage for the computation of the output concentrations. Results: We present a novel variational coupled model for the joint super-resolution and segmentation stage, and an automatic innovative image analysis for the diagnosis purpose. A specially designed Forward Backward-based numerical algorithm is introduced and its convergence is proved under mild conditions. We present results on a cheap prototype CMOS camera compared with the results of a more expensive CCD device, for the detection of the Dengue virus with a low-cost OLED light source. The combinationoftheCMOSsensorandthedevelopedpost-processingsoftwareallowstocorrectlyidentifythe different Dengue serotype using an automatized procedure. Conclusions: The results demonstrate that our diagnostic imaging system enables camera cost reduction up to 99%, at an acceptable diagnostic accuracy, with respect to the reference CCD-based camera system. The correct detection and identification of the Dengue serotypes has been confirmed by standard diagnostic methods (RT-PCR and ELISA)

Damiana, L., Serena, M., Melpignano, P., Loli Piccolomini, E., Luca, B. (2018). Image enhancement variational methods for Enabling Strong Cost Reduction in OLED-based Point-of-Care Immunofluorescent Diagnostic Systems. INTERNATIONAL JOURNAL FOR NUMERICAL METHODS IN BIOMEDICAL ENGINEERING, 34(3), 2932-2951 [10.1002/cnm.2932].

Image enhancement variational methods for Enabling Strong Cost Reduction in OLED-based Point-of-Care Immunofluorescent Diagnostic Systems

Damiana Lazzaro;Serena Morigi;E. Loli Piccolomini;Luca Benini
2018

Abstract

Objective:Immunofluorescence diagnostic systems cost is often dominated by high-sensitivity, low-noise CCD-based cameras which are used to acquire the fluorescence images. In this paper we investigate the use of low-cost CMOS sensors in a point-of-care immunofluorescence diagnostic application for the detection and discrimination of four different serotypes of the Dengue virus in a set of human samples. Methods: A two-phase post-processing software pipeline is proposed which consists in a first image enhancement stage for resolution increasing and segmentation, and a second diagnosis stage for the computation of the output concentrations. Results: We present a novel variational coupled model for the joint super-resolution and segmentation stage, and an automatic innovative image analysis for the diagnosis purpose. A specially designed Forward Backward-based numerical algorithm is introduced and its convergence is proved under mild conditions. We present results on a cheap prototype CMOS camera compared with the results of a more expensive CCD device, for the detection of the Dengue virus with a low-cost OLED light source. The combinationoftheCMOSsensorandthedevelopedpost-processingsoftwareallowstocorrectlyidentifythe different Dengue serotype using an automatized procedure. Conclusions: The results demonstrate that our diagnostic imaging system enables camera cost reduction up to 99%, at an acceptable diagnostic accuracy, with respect to the reference CCD-based camera system. The correct detection and identification of the Dengue serotypes has been confirmed by standard diagnostic methods (RT-PCR and ELISA)
2018
Damiana, L., Serena, M., Melpignano, P., Loli Piccolomini, E., Luca, B. (2018). Image enhancement variational methods for Enabling Strong Cost Reduction in OLED-based Point-of-Care Immunofluorescent Diagnostic Systems. INTERNATIONAL JOURNAL FOR NUMERICAL METHODS IN BIOMEDICAL ENGINEERING, 34(3), 2932-2951 [10.1002/cnm.2932].
Damiana, Lazzaro; Serena, Morigi; Melpignano, P.; Loli Piccolomini, E.; Luca, Benini
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/614467
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