The present study aimed to evaluate the impact of Raphanus sativus cv Sango sprout juice (SSJ) administration (75 mg/kg b.w. SSJ/day) on the brain lipidomic profile (fatty acid, sterols, cholesterol oxidation) of rats (non-genetic model) subjected to a high-fat (34% crude fat) dietary regimen. The SSJ did not affect the lipid infiltration (7.7–9.3%) and the fatty acid composition of the rat brain, which was mainly composed by unsaturated fatty acids (∼ 58%); however, the high-fat diet regimen significantly halved linoleic acid (LA). The high-fat diet also decreased (21.13 mg/g) the level of brain cholesterol with respect to the regular diet (4.5% crude fat) (23.83 mg/g); however, when the diet was shifted from high-fat to a regular regimen with or without SSJ supplementation, the levels of cholesterol significantly (p < 0.05) increased up to 30.46 mg/g of brain. The main oxysterols were 24(S)-hydroxycholesterol (24(S)-HC) and β-epoxycholesterol (β-EC). The high-fat diet led to the highest cholesterol oxidation (63.1 μg/g), increasing 27-hydroxycholesterol (27-HC) infiltration (0.24 μg/g rat brain) through the blood-brain barrier (BBB) compared to the regular diet (0.13 μg/g rat brain). On the other hand, when the diet was switched from high-fat to a regular regimen with SSJ supplementation, a significant reduction of 27-HC in the rat brain was found. Although 24-HC did not significantly change (p = 0.054), an increasing trend was observed when high-fat diet was supplied. The principal component analysis (PCA) revealed that SSJ was more active in counteracting cholesterol oxidation when supplied with the high-fat diet, due to inverse correlation with 24(S)-HC and 27-HC; however, further studies are needed to better understand which is the relationship between LA and cholesterol homeostasis in rat brain.

V. Cardenia, F. Vivarelli, S. Cirillo, M. Paolini, M. T. Rodriguez-Estrada, D. Canistro (2017). Dietary effects of Raphanus sativus cv Sango on lipid and oxysterols accumulation in rat brain: A lipidomic study on a non-genetic obesity model. CHEMISTRY AND PHYSICS OF LIPIDS, 207, 206-213 [10.1016/j.chemphyslip.2017.05.005].

Dietary effects of Raphanus sativus cv Sango on lipid and oxysterols accumulation in rat brain: A lipidomic study on a non-genetic obesity model

CARDENIA, VLADIMIRO;VIVARELLI, FABIO;CIRILLO, SILVIA;PAOLINI, MORENO;RODRIGUEZ ESTRADA, MARIA TERESA;CANISTRO, DONATELLA
2017

Abstract

The present study aimed to evaluate the impact of Raphanus sativus cv Sango sprout juice (SSJ) administration (75 mg/kg b.w. SSJ/day) on the brain lipidomic profile (fatty acid, sterols, cholesterol oxidation) of rats (non-genetic model) subjected to a high-fat (34% crude fat) dietary regimen. The SSJ did not affect the lipid infiltration (7.7–9.3%) and the fatty acid composition of the rat brain, which was mainly composed by unsaturated fatty acids (∼ 58%); however, the high-fat diet regimen significantly halved linoleic acid (LA). The high-fat diet also decreased (21.13 mg/g) the level of brain cholesterol with respect to the regular diet (4.5% crude fat) (23.83 mg/g); however, when the diet was shifted from high-fat to a regular regimen with or without SSJ supplementation, the levels of cholesterol significantly (p < 0.05) increased up to 30.46 mg/g of brain. The main oxysterols were 24(S)-hydroxycholesterol (24(S)-HC) and β-epoxycholesterol (β-EC). The high-fat diet led to the highest cholesterol oxidation (63.1 μg/g), increasing 27-hydroxycholesterol (27-HC) infiltration (0.24 μg/g rat brain) through the blood-brain barrier (BBB) compared to the regular diet (0.13 μg/g rat brain). On the other hand, when the diet was switched from high-fat to a regular regimen with SSJ supplementation, a significant reduction of 27-HC in the rat brain was found. Although 24-HC did not significantly change (p = 0.054), an increasing trend was observed when high-fat diet was supplied. The principal component analysis (PCA) revealed that SSJ was more active in counteracting cholesterol oxidation when supplied with the high-fat diet, due to inverse correlation with 24(S)-HC and 27-HC; however, further studies are needed to better understand which is the relationship between LA and cholesterol homeostasis in rat brain.
2017
V. Cardenia, F. Vivarelli, S. Cirillo, M. Paolini, M. T. Rodriguez-Estrada, D. Canistro (2017). Dietary effects of Raphanus sativus cv Sango on lipid and oxysterols accumulation in rat brain: A lipidomic study on a non-genetic obesity model. CHEMISTRY AND PHYSICS OF LIPIDS, 207, 206-213 [10.1016/j.chemphyslip.2017.05.005].
V. Cardenia; F. Vivarelli; S. Cirillo; M. Paolini; M. T. Rodriguez-Estrada; D. Canistro
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/610019
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