CRIS Current Research Information System
IRIS è la soluzione IT che facilita la raccolta e la gestione dei dati relativi alle attività e ai prodotti della ricerca. Fornisce a ricercatori, amministratori e valutatori gli strumenti per monitorare i risultati della ricerca, aumentarne la visibilità e allocare in modo efficace le risorse disponibili.
EnglishThe present study aimed to evaluate the impact of Raphanus sativus cv Sango sprout juice (SSJ) administration (75 mg/kg b.w. SSJ/day) on the brain lipidomic profile (fatty acid, sterols, cholesterol oxidation) of rats (non-genetic model) subjected to a high-fat (34% crude fat) dietary regimen. The SSJ did not affect the lipid infiltration (7.7–9.3%) and the fatty acid composition of the rat brain, which was mainly composed by unsaturated fatty acids (∼ 58%); however, the high-fat diet regimen significantly halved linoleic acid (LA). The high-fat diet also decreased (21.13 mg/g) the level of brain cholesterol with respect to the regular diet (4.5% crude fat) (23.83 mg/g); however, when the diet was shifted from high-fat to a regular regimen with or without SSJ supplementation, the levels of cholesterol significantly (p < 0.05) increased up to 30.46 mg/g of brain. The main oxysterols were 24(S)-hydroxycholesterol (24(S)-HC) and β-epoxycholesterol (β-EC). The high-fat diet led to the highest cholesterol oxidation (63.1 μg/g), increasing 27-hydroxycholesterol (27-HC) infiltration (0.24 μg/g rat brain) through the blood-brain barrier (BBB) compared to the regular diet (0.13 μg/g rat brain). On the other hand, when the diet was switched from high-fat to a regular regimen with SSJ supplementation, a significant reduction of 27-HC in the rat brain was found. Although 24-HC did not significantly change (p = 0.054), an increasing trend was observed when high-fat diet was supplied. The principal component analysis (PCA) revealed that SSJ was more active in counteracting cholesterol oxidation when supplied with the high-fat diet, due to inverse correlation with 24(S)-HC and 27-HC; however, further studies are needed to better understand which is the relationship between LA and cholesterol homeostasis in rat brain.
| Titolo: | Dietary effects of Raphanus sativus cv Sango on lipid and oxysterols accumulation in rat brain: A lipidomic study on a non-genetic obesity model | |
| Autore/i: | CARDENIA, VLADIMIRO; VIVARELLI, FABIO; CIRILLO, SILVIA; PAOLINI, MORENO; RODRIGUEZ ESTRADA, MARIA TERESA; CANISTRO, DONATELLA | |
| Autore/i Unibo: | ||
| Anno: | 2017 | |
| Rivista: | ||
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/j.chemphyslip.2017.05.005 | |
| Abstract: | The present study aimed to evaluate the impact of Raphanus sativus cv Sango sprout juice (SSJ) administration (75 mg/kg b.w. SSJ/day) on the brain lipidomic profile (fatty acid, sterols, cholesterol oxidation) of rats (non-genetic model) subjected to a high-fat (34% crude fat) dietary regimen. The SSJ did not affect the lipid infiltration (7.7–9.3%) and the fatty acid composition of the rat brain, which was mainly composed by unsaturated fatty acids (∼ 58%); however, the high-fat diet regimen significantly halved linoleic acid (LA). The high-fat diet also decreased (21.13 mg/g) the level of brain cholesterol with respect to the regular diet (4.5% crude fat) (23.83 mg/g); however, when the diet was shifted from high-fat to a regular regimen with or without SSJ supplementation, the levels of cholesterol significantly (p < 0.05) increased up to 30.46 mg/g of brain. The main oxysterols were 24(S)-hydroxycholesterol (24(S)-HC) and β-epoxycholesterol (β-EC). The high-fat diet led to the highest cholesterol oxidation (63.1 μg/g), increasing 27-hydroxycholesterol (27-HC) infiltration (0.24 μg/g rat brain) through the blood-brain barrier (BBB) compared to the regular diet (0.13 μg/g rat brain). On the other hand, when the diet was switched from high-fat to a regular regimen with SSJ supplementation, a significant reduction of 27-HC in the rat brain was found. Although 24-HC did not significantly change (p = 0.054), an increasing trend was observed when high-fat diet was supplied. The principal component analysis (PCA) revealed that SSJ was more active in counteracting cholesterol oxidation when supplied with the high-fat diet, due to inverse correlation with 24(S)-HC and 27-HC; however, further studies are needed to better understand which is the relationship between LA and cholesterol homeostasis in rat brain. | |
| Data stato definitivo: | 2017-11-04T15:07:44Z | |
| Appare nelle tipologie: | 1.01 Articolo in rivista |
File in questo prodotto:
Copyright © 2021