The maintenance of the endothelial function, challenged by oxidative stress and inflammation, is a fundamental target of preventive dietary strategies. The aim of this study was to investigate the protective activities of a wheat peptide belonging to the family of the "non-specific lipid transfer protein type 2" (nsLTP2). Results showed that HUVECs pretreatment with nsLTP2 significantly decreased both oxidative stress and cell damage induced by inflammation, and significantly counteracted the increased expression of adhesion molecules (VCAM-1, ICAM-1, E-selectin). Moreover, nsLTP2 increased the expression of HO-1, similarly to statins. As HO-1 has been shown to ameliorate inflammation in part through its ability to inhibit expression of endothelial adhesion molecules, experiments were also conducted using two HO-1 inhibitors, showing that the induced expression of HO-1 contributes to the anti-inflammatory activity exerted by nsLTP2. Taken together, obtained results suggest a nutraceutical role played by nsLTP2 in maintaining vascular health. This work was supported by BACCHUS project (FP7 European Commission Grant Agreement 312090)

Evaluation of the potential protective effect on vascular endothelium exerted by the peptide nsLTP2 identified in wheat.

LEONCINI, EMANUELA;PRATA, CECILIA;MASSACCESI, LUCA;MALAGUTI, MARCO;ANGELONI, CRISTINA;MAROTTI, ILARIA;BREGOLA, VALERIA;BOSI, SARA;DINELLI, GIOVANNI;HRELIA, SILVANA
2016

Abstract

The maintenance of the endothelial function, challenged by oxidative stress and inflammation, is a fundamental target of preventive dietary strategies. The aim of this study was to investigate the protective activities of a wheat peptide belonging to the family of the "non-specific lipid transfer protein type 2" (nsLTP2). Results showed that HUVECs pretreatment with nsLTP2 significantly decreased both oxidative stress and cell damage induced by inflammation, and significantly counteracted the increased expression of adhesion molecules (VCAM-1, ICAM-1, E-selectin). Moreover, nsLTP2 increased the expression of HO-1, similarly to statins. As HO-1 has been shown to ameliorate inflammation in part through its ability to inhibit expression of endothelial adhesion molecules, experiments were also conducted using two HO-1 inhibitors, showing that the induced expression of HO-1 contributes to the anti-inflammatory activity exerted by nsLTP2. Taken together, obtained results suggest a nutraceutical role played by nsLTP2 in maintaining vascular health. This work was supported by BACCHUS project (FP7 European Commission Grant Agreement 312090)
XIV FISV CONGRESS Rome, September 10th - 23rd 2016 Programme and Abstract - Sapienza University Rome
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Emanuela, Leoncini; Cecilia, Prata; Luca, Massaccesi; Marco, Malaguti; Cristina, Angeloni; Ilaria, Marotti; Valeria, Bregola; Sara, Bosi; Giovanni, Dinelli; Silvana, Hrelia
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11585/567446
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