A new CZE method was developed and compared with HPLC for the determination of (E)-10-hydroxy-2-decenoic acid (10-HDA) in commercial pure royal jelly (RJ) samples of different geographical origin. The results obtained with the CZE method were highly correlated with those of HPLC (p < 0.01). Under optimized conditions, CZE employed minimal amounts of a 50 mM tetraborate buffer as BGE, without the addition of any organic solvent, EOF or pH modifier. The new CZE method showed a wide linear response range (0.006-0.800 mg 10-HDA/ml) good sensitivity (LOD and LOQ were 0.002 and 0.004 mg/ml, respectively) and excellent instrumental repeatability with respect to migration time and peak area (RSD% < 2.0 for intraday assay and < 4.0 for interday assay). The 10-HDA content in pure RJ ranged from 0.8 to 3.2 g/100g of RJ and a significant difference (p < 0.05) was found between the Italian and the extra-European RJs average values: 2.5 and 1.5 g/100g of RJ respectively, according to the CZE data. The possibility of application of CZE for routine analyses on RJ and RJ based products to verify their authenticity was here highlighted.

Determination of (E)-10-hydroxy-2-decenoic acid content in pure royal jelly: a comparison between a new CZE method and HPLC

FERIOLI, FEDERICO;CABONI, MARIA
2007

Abstract

A new CZE method was developed and compared with HPLC for the determination of (E)-10-hydroxy-2-decenoic acid (10-HDA) in commercial pure royal jelly (RJ) samples of different geographical origin. The results obtained with the CZE method were highly correlated with those of HPLC (p < 0.01). Under optimized conditions, CZE employed minimal amounts of a 50 mM tetraborate buffer as BGE, without the addition of any organic solvent, EOF or pH modifier. The new CZE method showed a wide linear response range (0.006-0.800 mg 10-HDA/ml) good sensitivity (LOD and LOQ were 0.002 and 0.004 mg/ml, respectively) and excellent instrumental repeatability with respect to migration time and peak area (RSD% < 2.0 for intraday assay and < 4.0 for interday assay). The 10-HDA content in pure RJ ranged from 0.8 to 3.2 g/100g of RJ and a significant difference (p < 0.05) was found between the Italian and the extra-European RJs average values: 2.5 and 1.5 g/100g of RJ respectively, according to the CZE data. The possibility of application of CZE for routine analyses on RJ and RJ based products to verify their authenticity was here highlighted.
2007
F. Ferioli; G. L. Marcazzan; M. F. Caboni
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/37998
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