The content of sterol oxidation products (SOP) in ready-to eat infant foods was evaluated by thin-layer chromatography-gas chromatography (TLC-GC). Two different flavor (honey or fruits) liquid infant foods prepared with milk and cereals, were subjected to various storage periods (0, 2, 4, 7 and 9 months) at 25ºC. Lipids were extracted, subjected to cold saponification, purified by silica solid-phase, silylated and analyzed by GC. Their identification was further confirmed by TLC and GC-mass spectrometry (GC-MS). The main SOP found were the 7-keto derivatives of all sterols analyzed, being the 7-ketositosterol the most abundant one (0.33 mg/kg of sample). However, alpha- and beta-epoxy derivatives of both sitosterol and cholesterol, as well as cholestanetriol, were detected in the two types of samples. The extent of cholesterol oxidation (0.04%) was twice as much that of phytosterols (0.02%). However, no significant differences in the sterol and SOPs content (average value of 16.4 mg/100g sample and 1.29 mg/kg sample, respectively) of the two infant food formulations, were found. In addition, no significant effects of storage on the SOPs content of both infant food formulations, were detected.
Sterol oxidation in ready-to-eat infant foods / G. Garcia Llatas; L. Cercaci; M. T. Rodriguez-Estrada; M. J. Lagarda; G. Lercker. - STAMPA. - (2006), pp. 386-386. (Intervento presentato al convegno 4th EuroFed Lipid Congress. Oils, Fats and Lipids for a Healthier Future. The Need for Interdisciplinary Approaches tenutosi a University Complutense of Madrid, Madrid (Spain) nel 1-4 October 2006).
Sterol oxidation in ready-to-eat infant foods
CERCACI, LUISITO;RODRIGUEZ ESTRADA, MARIA TERESA;LERCKER, GIOVANNI
2006
Abstract
The content of sterol oxidation products (SOP) in ready-to eat infant foods was evaluated by thin-layer chromatography-gas chromatography (TLC-GC). Two different flavor (honey or fruits) liquid infant foods prepared with milk and cereals, were subjected to various storage periods (0, 2, 4, 7 and 9 months) at 25ºC. Lipids were extracted, subjected to cold saponification, purified by silica solid-phase, silylated and analyzed by GC. Their identification was further confirmed by TLC and GC-mass spectrometry (GC-MS). The main SOP found were the 7-keto derivatives of all sterols analyzed, being the 7-ketositosterol the most abundant one (0.33 mg/kg of sample). However, alpha- and beta-epoxy derivatives of both sitosterol and cholesterol, as well as cholestanetriol, were detected in the two types of samples. The extent of cholesterol oxidation (0.04%) was twice as much that of phytosterols (0.02%). However, no significant differences in the sterol and SOPs content (average value of 16.4 mg/100g sample and 1.29 mg/kg sample, respectively) of the two infant food formulations, were found. In addition, no significant effects of storage on the SOPs content of both infant food formulations, were detected.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
