Cardiac ischemia/reperfusion injury results in oxidative stress and poor physiological recovery. Episodes of hypoxia/reoxygenation (H/R) cause some subtle functional and structural alterations in sarcolemma, mithocondria, sarcoplasmic reticulum, nucleus, as well as cytoskeleton. In this report, by using cultured rat cardiomyocytes and laser confocal microscopy we have verified the possibility to counteract cytoskeleton alterations induced by H/R with the supplementation of an antioxidant agent, a green tea extract (GTE), and compared its effects to those of alpha-tocopherol. Moreover the effects of GTE on cell viability and cytosolic antioxidant activity have been evaluated. H/R induced myocardial damage occurs as histological alterations such as degeneration and disorganization of the cytoskeleton and loss of structural integrity of the nucleus. GTE supplementation increases cytosolic antioxidant activity and shows protective effects on cardiomyocyte cytoarchitecture and viability.

E. Pagnotta, N. Calonghi, S. Hrelia, L. Masotti, P.L. Biagi, C. Angeloni (2006). Green tea protects cytoskeleton from oxidative injury in cardiomyocytes. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 54, 10159-10163.

Green tea protects cytoskeleton from oxidative injury in cardiomyocytes.

PAGNOTTA, ELEONORA;CALONGHI, NATALIA;HRELIA, SILVANA;MASOTTI, LANFRANCO;BIAGI, PIERLUIGI;ANGELONI, CRISTINA
2006

Abstract

Cardiac ischemia/reperfusion injury results in oxidative stress and poor physiological recovery. Episodes of hypoxia/reoxygenation (H/R) cause some subtle functional and structural alterations in sarcolemma, mithocondria, sarcoplasmic reticulum, nucleus, as well as cytoskeleton. In this report, by using cultured rat cardiomyocytes and laser confocal microscopy we have verified the possibility to counteract cytoskeleton alterations induced by H/R with the supplementation of an antioxidant agent, a green tea extract (GTE), and compared its effects to those of alpha-tocopherol. Moreover the effects of GTE on cell viability and cytosolic antioxidant activity have been evaluated. H/R induced myocardial damage occurs as histological alterations such as degeneration and disorganization of the cytoskeleton and loss of structural integrity of the nucleus. GTE supplementation increases cytosolic antioxidant activity and shows protective effects on cardiomyocyte cytoarchitecture and viability.
2006
E. Pagnotta, N. Calonghi, S. Hrelia, L. Masotti, P.L. Biagi, C. Angeloni (2006). Green tea protects cytoskeleton from oxidative injury in cardiomyocytes. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 54, 10159-10163.
E. Pagnotta; N. Calonghi; S. Hrelia; L. Masotti; P.L. Biagi; C. Angeloni
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/33247
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 16
  • ???jsp.display-item.citation.isi??? 15
social impact