A Fast GC/MS method for plant sterols (PS) analysis was developed, using a capillary GC column coated with 5% diphenyl-polysiloxane. The main PS (beta-sitosterol, campesterol, stigmasterol, D5-avenasterol, campestanol and sitostanol) were well separated in 1.5 min, with a good peak resolution (R>1.4). Good repeatability (intraday RSD<7.3%, interday RSD<8.8%) and sensitivity (0.006-0.026 ng) were also achieved. Finally, the Fast GC/MS method for the determination of PS was tested on a PS-enriched food (milk). The analytical performance and significant reduction of the analysis time and consumables, demonstrates that Fast GC/MS could be also employed for the PS analysis in functional foods.

Analysis of plant sterols in enriched food by Fast gas chromatography/mass spectrometry

CARDENIA, VLADIMIRO;INCHINGOLO, RAFFAELLA;RODRIGUEZ ESTRADA, MARIA TERESA
2013

Abstract

A Fast GC/MS method for plant sterols (PS) analysis was developed, using a capillary GC column coated with 5% diphenyl-polysiloxane. The main PS (beta-sitosterol, campesterol, stigmasterol, D5-avenasterol, campestanol and sitostanol) were well separated in 1.5 min, with a good peak resolution (R>1.4). Good repeatability (intraday RSD<7.3%, interday RSD<8.8%) and sensitivity (0.006-0.026 ng) were also achieved. Finally, the Fast GC/MS method for the determination of PS was tested on a PS-enriched food (milk). The analytical performance and significant reduction of the analysis time and consumables, demonstrates that Fast GC/MS could be also employed for the PS analysis in functional foods.
Book of Abstracts- 3rd MS Food Day
220
221
V. Cardenia; R. Inchingolo; M. T. Rodriguez-Estrada
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/201542
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact