A large study centred on the authentication of origin (lato sensu) of European seabass and funded by the Italian Ministry of Agricultural, Food and Forestry Policies was set up with the aim of identifying the most cost-effective assemblage of analytical methods towards product authentication. The discriminatory power of each of those methods, when taken alone, was also assessed at an intermediate stage of the project. The part reported here explores the possibility of correctly identifying European seabass sources based exclusively on flesh multi-elemental analysis by ICP-AES. A total of 160 European seabass specimens were collected from 18 sources in the period November 2009 - December 2010. Aquacultured specimens (n = 115) were obtained from 13 farms (Italy 8, Greece 2, Turkey 2, Croatia 1), one of the Italian farms adopting quite a typical extensive production system in brackish lagoons named “vallicoltura”, the others predominantly the floating cage intensive system. Wild specimens (n = 45) were obtained from 5 main areas for seabass sourcing in Italy, 4 of which in the Mediterranean sea. Fish were received on ice and promptly filleted, skinned and deboned. The homogenised flesh samples, once microwave-digested, were individually analysed for six macro (Ca, K, Mg, Na, P, S) and twelve trace elements (As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Se, Zn) by ICP-AES. Supervised methods (quadratic discriminant analysis with leave-one-out crossvalidation) were performed on the whole data set, partial results from an exploratory round of statistical analyses being reported here. At this first attempt, elemental fingerprinting seemed not to be able to fully discriminate among the 18 European seabass sources. Slightly less than 40% of all the specimens was allocated to its own source (n = 63, 84% of which had been collected during the Autumn and Winter months). Slightly more than 62% of the wild specimens (28 out of 45) was allocated to the proper FAO fishing area. Significant differences between wild (W) and farmed (F) specimens were found in the content of K (W/F = 0.9625), Na (1.1272), P (0.9476), Cu (0.8680), Mn (0.8692), Se (1.2212), Zn (0.9378).
Silvi M., Guerrieri A., Remondini D., Trentini M., Rotolo M., Testi S., et al. (2011). Multi-elemental analysis by Inductively Coupled Plasma - Atomic Emission Spectroscopy (ICP-AES) of European seabass (Dicentrarchus labrax) from several sources. GOTHENBURG : Chalmers University of Technology.
Multi-elemental analysis by Inductively Coupled Plasma - Atomic Emission Spectroscopy (ICP-AES) of European seabass (Dicentrarchus labrax) from several sources
SILVI, MARINA;GUERRIERI, ALFONSINA;REMONDINI, DANIEL;TRENTINI, MASSIMO;ROTOLO, MAGDA;TESTI, SILVIA;PIRINI, MAURIZIO;BADIANI, ANNA
2011
Abstract
A large study centred on the authentication of origin (lato sensu) of European seabass and funded by the Italian Ministry of Agricultural, Food and Forestry Policies was set up with the aim of identifying the most cost-effective assemblage of analytical methods towards product authentication. The discriminatory power of each of those methods, when taken alone, was also assessed at an intermediate stage of the project. The part reported here explores the possibility of correctly identifying European seabass sources based exclusively on flesh multi-elemental analysis by ICP-AES. A total of 160 European seabass specimens were collected from 18 sources in the period November 2009 - December 2010. Aquacultured specimens (n = 115) were obtained from 13 farms (Italy 8, Greece 2, Turkey 2, Croatia 1), one of the Italian farms adopting quite a typical extensive production system in brackish lagoons named “vallicoltura”, the others predominantly the floating cage intensive system. Wild specimens (n = 45) were obtained from 5 main areas for seabass sourcing in Italy, 4 of which in the Mediterranean sea. Fish were received on ice and promptly filleted, skinned and deboned. The homogenised flesh samples, once microwave-digested, were individually analysed for six macro (Ca, K, Mg, Na, P, S) and twelve trace elements (As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Se, Zn) by ICP-AES. Supervised methods (quadratic discriminant analysis with leave-one-out crossvalidation) were performed on the whole data set, partial results from an exploratory round of statistical analyses being reported here. At this first attempt, elemental fingerprinting seemed not to be able to fully discriminate among the 18 European seabass sources. Slightly less than 40% of all the specimens was allocated to its own source (n = 63, 84% of which had been collected during the Autumn and Winter months). Slightly more than 62% of the wild specimens (28 out of 45) was allocated to the proper FAO fishing area. Significant differences between wild (W) and farmed (F) specimens were found in the content of K (W/F = 0.9625), Na (1.1272), P (0.9476), Cu (0.8680), Mn (0.8692), Se (1.2212), Zn (0.9378).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
