Preservation of acellular matrices represents a big challenge for the improvement of tissue engineering. In this work, a new method to preserve over time a decellularized esophageal scaffolds was explored. Dried and sterile acellular esophagi were obtained with a combined treatment of ethanol and a subsequent supercritical CO2 drying. Preservation of the extracellular matrix architecture, collagen content, and mechanical properties up to 6 months demonstrated the efficiency of the methodology with implications in natural scaffold storage. In vitro support of mesenchymal stem cells showed a promising indication to the further use of the technology in pre-clinical and clinical application.
Giobbe G. G., Zambon A., Vetralla M., Urbani L., Deguchi K., Pantano M. F., et al. (2018). Preservation over time of dried acellular esophageal matrix. BIOMEDICAL PHYSICS & ENGINEERING EXPRESS, 4(6), 1-9 [10.1088/2057-1976/aae4ff].
Preservation over time of dried acellular esophageal matrix
Zambon A.;
2018
Abstract
Preservation of acellular matrices represents a big challenge for the improvement of tissue engineering. In this work, a new method to preserve over time a decellularized esophageal scaffolds was explored. Dried and sterile acellular esophagi were obtained with a combined treatment of ethanol and a subsequent supercritical CO2 drying. Preservation of the extracellular matrix architecture, collagen content, and mechanical properties up to 6 months demonstrated the efficiency of the methodology with implications in natural scaffold storage. In vitro support of mesenchymal stem cells showed a promising indication to the further use of the technology in pre-clinical and clinical application.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
