Multipartite virus genomes are composed of two or more segments, each packaged into an independent viral particle. A potential advantage of multipartitism is the regulation of gene expression through changes in the segment copy number. Soil-borne beet necrotic yellow vein virus (BNYVV) is a typical example of multipartism, given its high number of genomic positive-sense RNAs (up to five). Here we analyse the relative frequencies of the four genomic RNAs of BNYVV type B during infection of different host plants (Chenopodium quinoa, Beta macrocarpa and Spinacia oleracea) and organs (leaves and roots). By successfully validating a two-step reverse-transcriptase digital droplet PCR protocol, we show that RNA1 and -2 genomic segments always replicate at low and comparable relative frequencies. In contrast, RNA3 and -4 accumulate with variable relative frequencies, resulting in distinct RNA1: RNA2: RNA3: RNA4 ratios, depending on the infected host species and organ.
Analysis of the relative frequencies of the multipartite BNYVV genomic RNAs in different plants and tissues / Dall'Ara M.; Guo Y.; Poli D.; Gilmer D.; Ratti C.. - In: JOURNAL OF GENERAL VIROLOGY. - ISSN 0022-1317. - STAMPA. - 105:1(2024), pp. 1-11. [10.1099/jgv.0.001950]
Analysis of the relative frequencies of the multipartite BNYVV genomic RNAs in different plants and tissues
Dall'Ara M.
Co-primo
;Guo Y.Co-primo
;Ratti C.
Ultimo
2024
Abstract
Multipartite virus genomes are composed of two or more segments, each packaged into an independent viral particle. A potential advantage of multipartitism is the regulation of gene expression through changes in the segment copy number. Soil-borne beet necrotic yellow vein virus (BNYVV) is a typical example of multipartism, given its high number of genomic positive-sense RNAs (up to five). Here we analyse the relative frequencies of the four genomic RNAs of BNYVV type B during infection of different host plants (Chenopodium quinoa, Beta macrocarpa and Spinacia oleracea) and organs (leaves and roots). By successfully validating a two-step reverse-transcriptase digital droplet PCR protocol, we show that RNA1 and -2 genomic segments always replicate at low and comparable relative frequencies. In contrast, RNA3 and -4 accumulate with variable relative frequencies, resulting in distinct RNA1: RNA2: RNA3: RNA4 ratios, depending on the infected host species and organ.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.