In vertebrates, neurotrophins and their receptors play a fundamental role in the central and peripheral nervous systems. Several studies reported that each neurotrophin/receptor signalling pathway can perform various functions during axon development, neuronal growth, and plasticity. Previous investigations in some fish species have identified neurotrophins and their receptors in the spinal cord under physiological conditions and after injuries, highlighting their potential role during regeneration. In our study, for the first time, we used an excellent animal model, the zebrafish (Danio rerio), to compare the mRNA localization patterns of neurotrophins and receptors in the spinal cord. We quantified the levels of mRNA using qPCR, and identified the transcription pattern of each neurotrophin/receptor pathway via in situ hybridization. Our data show that ngf/trka are the most transcribed members in the adult zebrafish spinal cord.

Transcription Pattern of Neurotrophic Factors and Their Receptors in Adult Zebrafish Spinal Cord / Pietro Cacialli; Serena Ricci; Maurizio Lazzari; Liliana Milani; Valeria Franceschini. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - ELETTRONICO. - 24:13(2023), pp. 10953-10969. [10.3390/ijms241310953]

Transcription Pattern of Neurotrophic Factors and Their Receptors in Adult Zebrafish Spinal Cord

Pietro Cacialli
Primo
;
Maurizio Lazzari;Liliana Milani;Valeria Franceschini
2023

Abstract

In vertebrates, neurotrophins and their receptors play a fundamental role in the central and peripheral nervous systems. Several studies reported that each neurotrophin/receptor signalling pathway can perform various functions during axon development, neuronal growth, and plasticity. Previous investigations in some fish species have identified neurotrophins and their receptors in the spinal cord under physiological conditions and after injuries, highlighting their potential role during regeneration. In our study, for the first time, we used an excellent animal model, the zebrafish (Danio rerio), to compare the mRNA localization patterns of neurotrophins and receptors in the spinal cord. We quantified the levels of mRNA using qPCR, and identified the transcription pattern of each neurotrophin/receptor pathway via in situ hybridization. Our data show that ngf/trka are the most transcribed members in the adult zebrafish spinal cord.
2023
Transcription Pattern of Neurotrophic Factors and Their Receptors in Adult Zebrafish Spinal Cord / Pietro Cacialli; Serena Ricci; Maurizio Lazzari; Liliana Milani; Valeria Franceschini. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - ELETTRONICO. - 24:13(2023), pp. 10953-10969. [10.3390/ijms241310953]
Pietro Cacialli; Serena Ricci; Maurizio Lazzari; Liliana Milani; Valeria Franceschini
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/933635
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