Gut microbiota plays a key role in the development and maintenance of heath on livestock and contributes to their productive efficiency. Although gut microbiota taxonomy characterization has been increasingly investigated, little is known about its functional characteristics and phenotyping. To fill this gap, Biolog EcoPlatesTM could be applied, however, little is known about its application on pig gut samples. However, presently, there is no information regarding the effect of the conservation including freezing and length of storage of these complex matrices for their subsequent analysis through the BiologTM EcoPlates technology. This study evaluated the use of Biolog EcoPlatesTM to characterize the metabolic potential and activity of the microbial community and to investigate the effect of 1) different storage conditions (fresh vs frozen) 2) optimal cell concentrations 3) specific length of storage conditions of pig faeces. Our study aimed to explore microbial communities’ metabolism in animal fecal samples contributing to investigate host animal physiology
Assessment of Biolog EcoplateTM method for functional metabolic diversity of aerotolerant pig fecal microbiota
Alice Checcucci;Diana Luise;Monica Modesto;Federico Correa;Paolo Bosi;Paola Mattarelli;Paolo Trevisi
2022
Abstract
Gut microbiota plays a key role in the development and maintenance of heath on livestock and contributes to their productive efficiency. Although gut microbiota taxonomy characterization has been increasingly investigated, little is known about its functional characteristics and phenotyping. To fill this gap, Biolog EcoPlatesTM could be applied, however, little is known about its application on pig gut samples. However, presently, there is no information regarding the effect of the conservation including freezing and length of storage of these complex matrices for their subsequent analysis through the BiologTM EcoPlates technology. This study evaluated the use of Biolog EcoPlatesTM to characterize the metabolic potential and activity of the microbial community and to investigate the effect of 1) different storage conditions (fresh vs frozen) 2) optimal cell concentrations 3) specific length of storage conditions of pig faeces. Our study aimed to explore microbial communities’ metabolism in animal fecal samples contributing to investigate host animal physiologyI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
