The enzymes involved in the biosynthetic pathway of L-leucine were studied in plasmid-negative and plasmid-positive clones derived from the RU 809 strain of the Bifidobacterium globosum species. The growth of plasmid-positive clones in synthetic medium required L-leucine. We have shown that no detectable activity of the β-isopropylmalate dehydrogenase enzyme was present in plasmid-positive clones, whereas detectable and significant activity of this enzyme was found in plasmid-negative clones. The lack of activity of the β-isopropylmalate dehydrogenase enzyme is considered responsible for the L-leucine auxotrophy in the plasmid-positive clones.

Mattarelli P., Biavati B. (1999). L-leucine auxotrophy in Bifidobacterium globosum. NEW MICROBIOLOGICA, 22(1), 73-76.

L-leucine auxotrophy in Bifidobacterium globosum

Mattarelli P.;Biavati B.
1999

Abstract

The enzymes involved in the biosynthetic pathway of L-leucine were studied in plasmid-negative and plasmid-positive clones derived from the RU 809 strain of the Bifidobacterium globosum species. The growth of plasmid-positive clones in synthetic medium required L-leucine. We have shown that no detectable activity of the β-isopropylmalate dehydrogenase enzyme was present in plasmid-positive clones, whereas detectable and significant activity of this enzyme was found in plasmid-negative clones. The lack of activity of the β-isopropylmalate dehydrogenase enzyme is considered responsible for the L-leucine auxotrophy in the plasmid-positive clones.
1999
Mattarelli P., Biavati B. (1999). L-leucine auxotrophy in Bifidobacterium globosum. NEW MICROBIOLOGICA, 22(1), 73-76.
Mattarelli P.; Biavati B.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/919931
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