The enzymes involved in the biosynthetic pathway of L-leucine were studied in plasmid-negative and plasmid-positive clones derived from the RU 809 strain of the Bifidobacterium globosum species. The growth of plasmid-positive clones in synthetic medium required L-leucine. We have shown that no detectable activity of the β-isopropylmalate dehydrogenase enzyme was present in plasmid-positive clones, whereas detectable and significant activity of this enzyme was found in plasmid-negative clones. The lack of activity of the β-isopropylmalate dehydrogenase enzyme is considered responsible for the L-leucine auxotrophy in the plasmid-positive clones.
Mattarelli P., Biavati B. (1999). L-leucine auxotrophy in Bifidobacterium globosum. NEW MICROBIOLOGICA, 22(1), 73-76.
L-leucine auxotrophy in Bifidobacterium globosum
Mattarelli P.;Biavati B.
1999
Abstract
The enzymes involved in the biosynthetic pathway of L-leucine were studied in plasmid-negative and plasmid-positive clones derived from the RU 809 strain of the Bifidobacterium globosum species. The growth of plasmid-positive clones in synthetic medium required L-leucine. We have shown that no detectable activity of the β-isopropylmalate dehydrogenase enzyme was present in plasmid-positive clones, whereas detectable and significant activity of this enzyme was found in plasmid-negative clones. The lack of activity of the β-isopropylmalate dehydrogenase enzyme is considered responsible for the L-leucine auxotrophy in the plasmid-positive clones.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
