Introduction: In the last decade, it has been discovered that allergen-bearing extracellular nanovesicles, termed “pollensomes”, are released by pollen during germination. These extracellular vesicles (EVs) may play an important role in pollen-pistil interaction during fertilization, stabilizing the secreted bioactive molecules and allowing long-distance signaling. However, the molecular composition and the biological role of these EVs are still unclear. The present study had two main aims: (I) to clarify whether pollen germination is needed to release pollensomes, or if they can be secreted also in high humidity conditions; and (II) to investigate the molecular features of pollensomes following the most recent guidelines for EVs isolation and identification. Methods: To do so, pollensomes were isolated from hydrated and germinated kiwi (Actinidia chinensis Planch.) pollen, and characterized using imaging techniques, immunoblotting, and proteomics. Results: These analyses revealed that only germinated kiwi pollen released detectable concentrations of nanoparticles compatible with small EVs for shape and protein content. Moreover, a plant homolog of ALIX, which is a well-recognized and accepted marker of small EVs and exosomes in mammals, was found in pollensomes. Discussion: The presence of this protein, along with other proteins involved in endocytosis, is consistent with the hypothesis that pollensomes could comprehend a prominent subpopulation of plant exosome-like vesicles.

Small extracellular vesicles released from germinated kiwi pollen (pollensomes) present characteristics similar to mammalian exosomes and carry a plant homolog of ALIX / Chiara Suanno, Elisa Tonoli, Enzo Fornari, Maria P. Savoca, Iris Aloisi, Luigi Parrotta, Claudia Faleri, Giampiero Cai, Clare Coveney, David J. Boocock, Elisabetta Verderio, Stefano Del Duca. - In: FRONTIERS IN PLANT SCIENCE. - ISSN 1664-462X. - ELETTRONICO. - 14:(2023), pp. 1090026.1-1090026.16. [10.3389/fpls.2023.1090026]

Small extracellular vesicles released from germinated kiwi pollen (pollensomes) present characteristics similar to mammalian exosomes and carry a plant homolog of ALIX

Chiara Suanno
Primo
;
Iris Aloisi
;
Luigi Parrotta;Elisabetta Verderio
Penultimo
;
Stefano Del Duca
Ultimo
2023

Abstract

Introduction: In the last decade, it has been discovered that allergen-bearing extracellular nanovesicles, termed “pollensomes”, are released by pollen during germination. These extracellular vesicles (EVs) may play an important role in pollen-pistil interaction during fertilization, stabilizing the secreted bioactive molecules and allowing long-distance signaling. However, the molecular composition and the biological role of these EVs are still unclear. The present study had two main aims: (I) to clarify whether pollen germination is needed to release pollensomes, or if they can be secreted also in high humidity conditions; and (II) to investigate the molecular features of pollensomes following the most recent guidelines for EVs isolation and identification. Methods: To do so, pollensomes were isolated from hydrated and germinated kiwi (Actinidia chinensis Planch.) pollen, and characterized using imaging techniques, immunoblotting, and proteomics. Results: These analyses revealed that only germinated kiwi pollen released detectable concentrations of nanoparticles compatible with small EVs for shape and protein content. Moreover, a plant homolog of ALIX, which is a well-recognized and accepted marker of small EVs and exosomes in mammals, was found in pollensomes. Discussion: The presence of this protein, along with other proteins involved in endocytosis, is consistent with the hypothesis that pollensomes could comprehend a prominent subpopulation of plant exosome-like vesicles.
2023
Small extracellular vesicles released from germinated kiwi pollen (pollensomes) present characteristics similar to mammalian exosomes and carry a plant homolog of ALIX / Chiara Suanno, Elisa Tonoli, Enzo Fornari, Maria P. Savoca, Iris Aloisi, Luigi Parrotta, Claudia Faleri, Giampiero Cai, Clare Coveney, David J. Boocock, Elisabetta Verderio, Stefano Del Duca. - In: FRONTIERS IN PLANT SCIENCE. - ISSN 1664-462X. - ELETTRONICO. - 14:(2023), pp. 1090026.1-1090026.16. [10.3389/fpls.2023.1090026]
Chiara Suanno, Elisa Tonoli, Enzo Fornari, Maria P. Savoca, Iris Aloisi, Luigi Parrotta, Claudia Faleri, Giampiero Cai, Clare Coveney, David J. Boocock, Elisabetta Verderio, Stefano Del Duca
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Descrizione: Small extracellular vesicles released from germinated kiwi pollen (pollensomes) present characteristics similar to mammalian exosomes and carry a plant homolog of ALIX
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/913056
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