Earthworm coelomocytes (leukocytes) effect cytotoxicity at significantly high levels against the NK-sensitive, human tumor cell line, K562, and the NK-resistant targets (U937, BSM, CEM). By cytofluorimetric analyses using mouse anti-human monoclonal antibodies and by morphological evaluations, two types of coelomocytes were identified: (1) small (8-11 micron) electron-dense cells (SC): CD11a+, CD45RA+, CD45RO+, CDw49b+, CD54+, beta 2-m+ and Thy-1+; (2) large (12-15 micron) electron-lucent cells (LC) that are negative for these markers. Both cell types were negative for other CD and MHC class I and class II markers. SC were active during recognition, rapidly binding to targets; LC were phagocytic. Release of 51Cr revealed rapid, significant, and equal levels of killing at 4 degrees, 20 degrees, and 37 degrees C. We propose that primitive NK-like activity appeared early in evolution.

G2.1 Earthworm leukocytes (CD11a+, CD45RA+, ß2-m+, Thy-1+) can kill human K562 tumor cell targets / Cooper E.L.; Suzuki M.M.; Cossarizza A.; Salvioli S.; Capri M.; Quaglino D.; Pasquali-Ronchetti I.; Franceschi C.. - In: DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY. - ISSN 0145-305X. - STAMPA. - 18:1(1994), pp. 174-182. [10.1016/0145-305X(94)90167-8]

G2.1 Earthworm leukocytes (CD11a+, CD45RA+, ß2-m+, Thy-1+) can kill human K562 tumor cell targets

Salvioli S.;Capri M.;Franceschi C.
1994

Abstract

Earthworm coelomocytes (leukocytes) effect cytotoxicity at significantly high levels against the NK-sensitive, human tumor cell line, K562, and the NK-resistant targets (U937, BSM, CEM). By cytofluorimetric analyses using mouse anti-human monoclonal antibodies and by morphological evaluations, two types of coelomocytes were identified: (1) small (8-11 micron) electron-dense cells (SC): CD11a+, CD45RA+, CD45RO+, CDw49b+, CD54+, beta 2-m+ and Thy-1+; (2) large (12-15 micron) electron-lucent cells (LC) that are negative for these markers. Both cell types were negative for other CD and MHC class I and class II markers. SC were active during recognition, rapidly binding to targets; LC were phagocytic. Release of 51Cr revealed rapid, significant, and equal levels of killing at 4 degrees, 20 degrees, and 37 degrees C. We propose that primitive NK-like activity appeared early in evolution.
1994
G2.1 Earthworm leukocytes (CD11a+, CD45RA+, ß2-m+, Thy-1+) can kill human K562 tumor cell targets / Cooper E.L.; Suzuki M.M.; Cossarizza A.; Salvioli S.; Capri M.; Quaglino D.; Pasquali-Ronchetti I.; Franceschi C.. - In: DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY. - ISSN 0145-305X. - STAMPA. - 18:1(1994), pp. 174-182. [10.1016/0145-305X(94)90167-8]
Cooper E.L.; Suzuki M.M.; Cossarizza A.; Salvioli S.; Capri M.; Quaglino D.; Pasquali-Ronchetti I.; Franceschi C.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/903141
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