Gastrointestinal stromal tumors (GISTs) harboring mutations in the PDGFRA gene occur in only about 5-7% of patients. The most common PDGFRA mutation is exon 18 D842V, which is correlated with specific clinico-pathological features compared to the other PDGFRA mutated GISTs. Herein, we present a miRNA expression profile comparison of PDGFRA D842V mutant GISTs and PDGFRA with mutations other than D842V (non-D842V). miRNA expression profiling was carried out on 10 patients using a TLDA miRNA array. Then, miRNA expression was followed by bioinformatic analysis aimed at evaluating differential expression, pathway enrichment, and miRNA-mRNA networks. We highlighted 24 differentially expressed miRNAs between D842V and non-D842V GIST patients. Pathway enrichment analysis showed that deregulated miRNAs targeted genes that are mainly involved in the immune response pathways. The miRNA-mRNA networks highlighted a signature of miRNAs/mRNA that could explain the indolent behavior of the D842V mutated GIST. The results highlighted a different miRNA fingerprint in PDGFRA D842V GISTs compared to non-D842Vmutated patients, which could explain the different biological behavior of this GIST subset.

Ravegnini, G., Nannini, M., Indio, V., Serrano, C., Gorini, F., Astolfi, A., et al. (2022). miRNA Expression May Have Implications for Immunotherapy in PDGFRA Mutant GISTs. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 23(20), 12248-12258 [10.3390/ijms232012248].

miRNA Expression May Have Implications for Immunotherapy in PDGFRA Mutant GISTs

Ravegnini, Gloria;Nannini, Margherita;Indio, Valentina;Gorini, Francesca;Astolfi, Annalisa;Di Vito, Aldo;Morroni, Fabiana;Pantaleo, Maria Abbondanza;Hrelia, Patrizia;Angelini, Sabrina
2022

Abstract

Gastrointestinal stromal tumors (GISTs) harboring mutations in the PDGFRA gene occur in only about 5-7% of patients. The most common PDGFRA mutation is exon 18 D842V, which is correlated with specific clinico-pathological features compared to the other PDGFRA mutated GISTs. Herein, we present a miRNA expression profile comparison of PDGFRA D842V mutant GISTs and PDGFRA with mutations other than D842V (non-D842V). miRNA expression profiling was carried out on 10 patients using a TLDA miRNA array. Then, miRNA expression was followed by bioinformatic analysis aimed at evaluating differential expression, pathway enrichment, and miRNA-mRNA networks. We highlighted 24 differentially expressed miRNAs between D842V and non-D842V GIST patients. Pathway enrichment analysis showed that deregulated miRNAs targeted genes that are mainly involved in the immune response pathways. The miRNA-mRNA networks highlighted a signature of miRNAs/mRNA that could explain the indolent behavior of the D842V mutated GIST. The results highlighted a different miRNA fingerprint in PDGFRA D842V GISTs compared to non-D842Vmutated patients, which could explain the different biological behavior of this GIST subset.
2022
Ravegnini, G., Nannini, M., Indio, V., Serrano, C., Gorini, F., Astolfi, A., et al. (2022). miRNA Expression May Have Implications for Immunotherapy in PDGFRA Mutant GISTs. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 23(20), 12248-12258 [10.3390/ijms232012248].
Ravegnini, Gloria; Nannini, Margherita; Indio, Valentina; Serrano, Cesar; Gorini, Francesca; Astolfi, Annalisa; Di Vito, Aldo; Morroni, Fabiana; Panta...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/900473
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