Five SARS-CoV-2-positive samples showed N-gene drop-out with a RT-PCR multiplex test. WGS found all samples to harbor a deletion in the same region of the N gene, which is likely to impair the efficiency of amplification. This highlights the need for a continued surveillance of viral evolution and diagnostic test performance.
A deletion in the N gene may cause diagnostic escape in SARS-CoV-2 samples / Zannoli S.; Dirani G.; Taddei F.; Gatti G.; Poggianti I.; Denicolo A.; Arfilli V.; Manera M.; Mancini A.; Battisti A.; Sambri V.. - In: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE. - ISSN 0732-8893. - STAMPA. - 102:1(2022), pp. 115540.115540-115540.115542. [10.1016/j.diagmicrobio.2021.115540]
A deletion in the N gene may cause diagnostic escape in SARS-CoV-2 samples
Zannoli S.;Dirani G.;Gatti G.;Arfilli V.;Battisti A.;Sambri V.
2022
Abstract
Five SARS-CoV-2-positive samples showed N-gene drop-out with a RT-PCR multiplex test. WGS found all samples to harbor a deletion in the same region of the N gene, which is likely to impair the efficiency of amplification. This highlights the need for a continued surveillance of viral evolution and diagnostic test performance.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.