Individual cells in the same induced pluripotent stem cell (iPSC)-derived clones can exhibit large heterogeneity. In this Comment, Carelli et al. discuss emerging evidence implicating variants in mitochondrial DNA, and highlight the need for routine screening of iPSCs.Single-cell analyses in recent years have shown major differences in the transcriptome between individual cells in the same induced pluripotent stem cell-derived clones. Although these differences are in part attributable to genetic and epigenetic modifications of the nuclear genome, emerging evidence suggests that variants in mitochondrial DNA also play a pivotal role.
Carelli V., Hirano M., Enriquez J.A., Chinnery P.F. (2022). Implications of mitochondrial DNA mutations in human induced pluripotent stem cells. NATURE REVIEWS GENETICS, 23(2), 69-70 [10.1038/s41576-021-00430-z].
Implications of mitochondrial DNA mutations in human induced pluripotent stem cells
Carelli V.;
2022
Abstract
Individual cells in the same induced pluripotent stem cell (iPSC)-derived clones can exhibit large heterogeneity. In this Comment, Carelli et al. discuss emerging evidence implicating variants in mitochondrial DNA, and highlight the need for routine screening of iPSCs.Single-cell analyses in recent years have shown major differences in the transcriptome between individual cells in the same induced pluripotent stem cell-derived clones. Although these differences are in part attributable to genetic and epigenetic modifications of the nuclear genome, emerging evidence suggests that variants in mitochondrial DNA also play a pivotal role.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
