The use of stem cells for regenerative applications and immunomodulatory effect is in-creasing. Amniotic epithelial cells (AECs) possess embryonic‐like proliferation ability and multipo-tent differentiation potential. Despite the simple isolation procedure, inter‐individual variability and different isolation steps can cause differences in isolation yield and cell proliferation ability, compromising reproducibility observations among centers and further applications. We investi-gated the use of a new technology as a diagnostic tool for quality control on stem cell isolation. The instrument label‐free separates cells based on their physical characteristics and, thanks to a micro-camera, generates a live fractogram, the fingerprint of the sample. Eight amniotic membranes were processed by trypsin enzymatic treatment and immediately analysed. Two types of profile were generated: a monomodal and a bimodal curve. The first one represented the unsuccessful isolation with all recovered cell not attaching to the plate; while for the second type, the isolation process was successful, but we discovered that only cells in the second peak were alive and resulted adherent. We optimized a Quality Control (QC) method to define the success of AEC isolation using the frac-togram generated. This predictive outcome is an interesting tool for laboratories and cell banks that isolate and cryopreserve fetal annex stem cells for research and future clinical applications.

A new predictive technology for perinatal stem cell isolation suited for cell therapy approaches / Zia S.; Martini G.; Pizzuti V.; Maggio A.; Simonazzi G.; Reschiglian P.; Bonsi L.; Alviano F.; Roda B.; Zattoni A.. - In: MICROMACHINES. - ISSN 2072-666X. - ELETTRONICO. - 12:7(2021), pp. 782-796. [10.3390/mi12070782]

A new predictive technology for perinatal stem cell isolation suited for cell therapy approaches

Martini G.
Formal Analysis
;
Pizzuti V.
Formal Analysis
;
Maggio A.
Formal Analysis
;
Simonazzi G.
Methodology
;
Reschiglian P.
Conceptualization
;
Bonsi L.
Conceptualization
;
Alviano F.
Conceptualization
;
Roda B.
Penultimo
Conceptualization
;
Zattoni A.
Ultimo
Methodology
2021

Abstract

The use of stem cells for regenerative applications and immunomodulatory effect is in-creasing. Amniotic epithelial cells (AECs) possess embryonic‐like proliferation ability and multipo-tent differentiation potential. Despite the simple isolation procedure, inter‐individual variability and different isolation steps can cause differences in isolation yield and cell proliferation ability, compromising reproducibility observations among centers and further applications. We investi-gated the use of a new technology as a diagnostic tool for quality control on stem cell isolation. The instrument label‐free separates cells based on their physical characteristics and, thanks to a micro-camera, generates a live fractogram, the fingerprint of the sample. Eight amniotic membranes were processed by trypsin enzymatic treatment and immediately analysed. Two types of profile were generated: a monomodal and a bimodal curve. The first one represented the unsuccessful isolation with all recovered cell not attaching to the plate; while for the second type, the isolation process was successful, but we discovered that only cells in the second peak were alive and resulted adherent. We optimized a Quality Control (QC) method to define the success of AEC isolation using the frac-togram generated. This predictive outcome is an interesting tool for laboratories and cell banks that isolate and cryopreserve fetal annex stem cells for research and future clinical applications.
2021
A new predictive technology for perinatal stem cell isolation suited for cell therapy approaches / Zia S.; Martini G.; Pizzuti V.; Maggio A.; Simonazzi G.; Reschiglian P.; Bonsi L.; Alviano F.; Roda B.; Zattoni A.. - In: MICROMACHINES. - ISSN 2072-666X. - ELETTRONICO. - 12:7(2021), pp. 782-796. [10.3390/mi12070782]
Zia S.; Martini G.; Pizzuti V.; Maggio A.; Simonazzi G.; Reschiglian P.; Bonsi L.; Alviano F.; Roda B.; Zattoni A.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/863185
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