Cardiomyocyte loss through apoptosis has been reported in a variety of cardiovascular diseases. Being a highly regulated cell death program its inhibition is cardioprotective. We previously demonstrated that sulforaphane (SF), present in many Cruciferous vegetables, boosts cell defence system acting as an indirect antioxidant. The aim of this study was to evaluate SF ability to modulate pro and anti-apoptotic proteins resulting in the inhibition of H2O2-induced apoptosis. SF treatment was able to protect cells against oxidative damage increasing cell viability, and decreasing apoptosis markers like DNA fragmentation and PS exposure. Accordingly, SF reduced bax translocation to mitochondria, cytochrome C release and caspase 3 activation in comparison to untreated cells. Specific protein kinases inhibitors revealed that SF protection is mainly mediated by Akt activation. Taken together these results show that a reduction in apoptotic cell death contributes to cardioprotection, suggesting SF as a promising nutraceutical in the prevention of oxidative stress related cardiac diseases.

Sulforaphane as an antiapoptotic nutraceutical in cardiac cells

LEONCINI, EMANUELA;ANGELONI, CRISTINA;MALAGUTI, MARCO;MOTORI, ELISA;HRELIA, SILVANA
2009

Abstract

Cardiomyocyte loss through apoptosis has been reported in a variety of cardiovascular diseases. Being a highly regulated cell death program its inhibition is cardioprotective. We previously demonstrated that sulforaphane (SF), present in many Cruciferous vegetables, boosts cell defence system acting as an indirect antioxidant. The aim of this study was to evaluate SF ability to modulate pro and anti-apoptotic proteins resulting in the inhibition of H2O2-induced apoptosis. SF treatment was able to protect cells against oxidative damage increasing cell viability, and decreasing apoptosis markers like DNA fragmentation and PS exposure. Accordingly, SF reduced bax translocation to mitochondria, cytochrome C release and caspase 3 activation in comparison to untreated cells. Specific protein kinases inhibitors revealed that SF protection is mainly mediated by Akt activation. Taken together these results show that a reduction in apoptotic cell death contributes to cardioprotection, suggesting SF as a promising nutraceutical in the prevention of oxidative stress related cardiac diseases.
2009
54th national meeting of the Italian Society of Biochemistry and Molecular Biology
194
194
E. Leoncini; C. Angeloni; M. Malaguti; E. Motori; S. Hrelia.
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/86291
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact