A novel and rapid realtime polymerase chain reaction (PCR) molecular approach for the detection of Pseudomonas aeruginosa, P. fluorescens and P. putida in biofilms in sheep dairy farms is described. This strategy is based on early detection of the subsequent rate: Pseudomonas vs. total bacteria in biofilm specimens using particular bi-functional FRET oligonucleotide probes named DUAL-FRET.
Orru G, Oggioni MR, Mandas D, Melis E, Trappetti C, Braina V, et al. (2009). Pseudomonas spp. isolated in diary herds. A new protocol with PCR real time by using “DUAL FRET” probes for ovine mastitis. LARGE ANIMALS REVIEW, 15(2), 65-70.
Pseudomonas spp. isolated in diary herds. A new protocol with PCR real time by using “DUAL FRET” probes for ovine mastitis
Oggioni MR;
2009
Abstract
A novel and rapid realtime polymerase chain reaction (PCR) molecular approach for the detection of Pseudomonas aeruginosa, P. fluorescens and P. putida in biofilms in sheep dairy farms is described. This strategy is based on early detection of the subsequent rate: Pseudomonas vs. total bacteria in biofilm specimens using particular bi-functional FRET oligonucleotide probes named DUAL-FRET.File in questo prodotto:
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