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EnglishThe synthesisandtranslocationofthediaminecadaverineduringsoybean(Glycinemax L. Meercv.Sakai) germinationwerestudiedusing 15N-labelledlysine(thecadaverineprecursor)and 15N-labelledcadaverine, bothunderlight/dark(12h/12h)andtotaldarkgerminatingconditions. 15N-cadaverineandnon-labelled polyaminesweresimultaneouslydetectedusingionsprayionizationemassspectrometry.Both 15N-cadaverineand 15N-lysineweretakenupbysoybean. 15N-lysinewastransportedtotheshootandroot and convertedinto 15N-cadaverine,whereasrelativelylittle 15N-cadaverinewasformedfrom 15N-lysine in thecotyledon.Theacropetaltranslocationof 15N-cadaverinefromthecotyledontotheshootseemed to predominateoverbasipetaltransporttotheroot.Althoughnoother 15N-derivatisedpolyamineswere found,supplyingexogenous 15N-lysineseemedtoindirectlyaffectthemetabolismof 14N putrescine, spermidineandspermine,whilenosignificanteffectwasdetectedaftersupplying 15N-cadaverine
| Titolo: | Cadaverine turnover in soybean seedlings using 15N-labelled lysine and cadaverine |
| Autore/i: | M. Ohe; H. Sasaki; M. Niitsu; BAGNI, NELLO; TASSONI, ANNALISA; S. Matsuzaki |
| Autore/i Unibo: | |
| Anno: | 2010 |
| Rivista: | |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/j.plaphy.2010.01.018 |
| Abstract: | The synthesisandtranslocationofthediaminecadaverineduringsoybean(Glycinemax L. Meercv.Sakai) germinationwerestudiedusing 15N-labelledlysine(thecadaverineprecursor)and 15N-labelledcadaverine, bothunderlight/dark(12h/12h)andtotaldarkgerminatingconditions. 15N-cadaverineandnon-labelled polyaminesweresimultaneouslydetectedusingionsprayionizationemassspectrometry.Both 15N-cadaverineand 15N-lysineweretakenupbysoybean. 15N-lysinewastransportedtotheshootandroot and convertedinto 15N-cadaverine,whereasrelativelylittle 15N-cadaverinewasformedfrom 15N-lysine in thecotyledon.Theacropetaltranslocationof 15N-cadaverinefromthecotyledontotheshootseemed to predominateoverbasipetaltransporttotheroot.Althoughnoother 15N-derivatisedpolyamineswere found,supplyingexogenous 15N-lysineseemedtoindirectlyaffectthemetabolismof 14N putrescine, spermidineandspermine,whilenosignificanteffectwasdetectedaftersupplying 15N-cadaverine |
| Data prodotto definitivo in UGOV: | 2010-09-29 10:45:08 |
| Data stato definitivo: | 2015-12-24T12:28:44Z |
| Appare nelle tipologie: | 1.01 Articolo in rivista |
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