The use of 2,5-dimethyl-1H-pyrrole-3,4-dicarbaldehyde as a precolumn derivatization reagent for LC analysis of amino acids is proposed. The compound reacts at mild conditions (10 min at ambient temperature) with primary amino groups. The derivatization conditions were optimised by considering different parameters (temperature, pH and reagent concentration) using L-Val. The reaction was found to be quantitative. The synthesized L-Val derivative was characterized by 1H-NMR and UV, whereas the adducts obtained by derivatization of 19 amino acids were separated by LC under reversed-phase conditions and detected at λ=320 nm. The method was applied successfully to the quali-quantitative analysis of commercial polyaminoacid preparations.
R.Gatti, M.G.Gioia, A.Leoni, A.Andreani (2010). 2,5-Dimethyl-1H-pyrrole-3,4-dicarbaldehyde as a precolumn derivatization reagent for HPLC/UV detection of amino acids. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 53, 207-211 [10.1016/j.jpba.2009.12.031].
2,5-Dimethyl-1H-pyrrole-3,4-dicarbaldehyde as a precolumn derivatization reagent for HPLC/UV detection of amino acids
GATTI, RITA;GIOIA, MARIA GRAZIA;LEONI, ALBERTO;ANDREANI, ALDO
2010
Abstract
The use of 2,5-dimethyl-1H-pyrrole-3,4-dicarbaldehyde as a precolumn derivatization reagent for LC analysis of amino acids is proposed. The compound reacts at mild conditions (10 min at ambient temperature) with primary amino groups. The derivatization conditions were optimised by considering different parameters (temperature, pH and reagent concentration) using L-Val. The reaction was found to be quantitative. The synthesized L-Val derivative was characterized by 1H-NMR and UV, whereas the adducts obtained by derivatization of 19 amino acids were separated by LC under reversed-phase conditions and detected at λ=320 nm. The method was applied successfully to the quali-quantitative analysis of commercial polyaminoacid preparations.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
