Tattooing is an ancient art and is still widely practiced all over the world. Since the biocompatibility of tattoo dyes has not been well researched, we studied the toxicity of a commercial tattoo ink, commonly used in tattoo lab and esthetic centers, on human fibroblasts. To test cell viability, MTT assays were carried out and scanning electron microscopy to visualize changes in the cell surface after the dye exposure was performed. A possible influence of the pigment on the expression of procollagen alpha 1 type I protein was visualized by western blotting analysis. The results showed a reduction in cell viability, and electron microscopy demonstrated an unmodified cell surface completely covered by pigment particles. Western blotting analysis demonstrated a clear interference of the pigment on the expression of procollagen alpha 1 type I protein. These data demonstrated that the commercial tattoo dye has a time-dependent effect on protein expression. A possible connection of the influence of the tattoo ink with clinical effects is discussed.

Influence of a commercial tattoo ink on protein production in human fibroblasts.

FALCONI, MIRELLA;TETI, GABRIELLA;ZAGO, MICHELA;GALANZI, ANGELA;BRESCHI, LORENZO;PELOTTI, SUSI;RUGGERI, ALESSANDRA;MAZZOTTI, GIOVANNI
2009

Abstract

Tattooing is an ancient art and is still widely practiced all over the world. Since the biocompatibility of tattoo dyes has not been well researched, we studied the toxicity of a commercial tattoo ink, commonly used in tattoo lab and esthetic centers, on human fibroblasts. To test cell viability, MTT assays were carried out and scanning electron microscopy to visualize changes in the cell surface after the dye exposure was performed. A possible influence of the pigment on the expression of procollagen alpha 1 type I protein was visualized by western blotting analysis. The results showed a reduction in cell viability, and electron microscopy demonstrated an unmodified cell surface completely covered by pigment particles. Western blotting analysis demonstrated a clear interference of the pigment on the expression of procollagen alpha 1 type I protein. These data demonstrated that the commercial tattoo dye has a time-dependent effect on protein expression. A possible connection of the influence of the tattoo ink with clinical effects is discussed.
ARCHIVES OF DERMATOLOGICAL RESEARCH
Falconi M; Teti G; Zago M; Galanzi A; Breschi L; Pelotti S; Ruggeri A; Mazzotti G.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11585/81245
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