Background and Objectives. To investigate the effects of age, sex and vitamin status on total plasma homocysteine (tHCy), both after fasting (FtHCy) and two hours post-methionine load (PML-tHCy). The secondary aim was to determine the reference values for FtHCy and PML-tHCy. .Design and Methods. A cohort of apparently healthy volunteers underwent blood sampling for FtHCy, PML-tHCy, creatinine, serum folate, vitamin B12 and vitamin B6 (pyridoxal-5-phosphate, PLP).Results. In 147 subjects (M/F= 82/65, age range: 14-94 years), FtHCy was significantly higher in men than in women. In men, age and folate levels explained 20.5% and 19.0% of FtHCy variance, respectively. In women, age and vitamin B12 accounted for 22.6% and 17.8% of FtHCy variance, respectively. PML-tHCy was similar in men and women, PML-tHCy was negatively correlated with folate in both sexes, and with vitamin B12 and age in women only. Folate accounted for 20% of the variance of PML-tHCy in men, while in women vitamin B12 and PLP explained 40% and 20% of variance of PML-tHCy, respectively, The reference values of FtHCy and PML-tHCy were: 19.63 and 40.18 mumol/L, respectively, for men under 45 years, 14.26 and 28.31 mumol/L, respectively, for women under 45 years, 28.38 and 36.48 mumol/L for men above 45 years, and 22.49 and 44.06 mumol/L for women above 45 years.Interpretation and Conclusions. Age, gender and vitamin status influence both FtHCy and PML-tHCy in normal subjects. Reference values should be calculated according to age and sex. (C) 2002, Ferrata Storti Foundation.
Influence of age, sex and vitamin status on fasting and post-methionine load plasma homocysteine levels
Cosmi, B;Palareti, G;Coccheri, S
2002
Abstract
Background and Objectives. To investigate the effects of age, sex and vitamin status on total plasma homocysteine (tHCy), both after fasting (FtHCy) and two hours post-methionine load (PML-tHCy). The secondary aim was to determine the reference values for FtHCy and PML-tHCy. .Design and Methods. A cohort of apparently healthy volunteers underwent blood sampling for FtHCy, PML-tHCy, creatinine, serum folate, vitamin B12 and vitamin B6 (pyridoxal-5-phosphate, PLP).Results. In 147 subjects (M/F= 82/65, age range: 14-94 years), FtHCy was significantly higher in men than in women. In men, age and folate levels explained 20.5% and 19.0% of FtHCy variance, respectively. In women, age and vitamin B12 accounted for 22.6% and 17.8% of FtHCy variance, respectively. PML-tHCy was similar in men and women, PML-tHCy was negatively correlated with folate in both sexes, and with vitamin B12 and age in women only. Folate accounted for 20% of the variance of PML-tHCy in men, while in women vitamin B12 and PLP explained 40% and 20% of variance of PML-tHCy, respectively, The reference values of FtHCy and PML-tHCy were: 19.63 and 40.18 mumol/L, respectively, for men under 45 years, 14.26 and 28.31 mumol/L, respectively, for women under 45 years, 28.38 and 36.48 mumol/L for men above 45 years, and 22.49 and 44.06 mumol/L for women above 45 years.Interpretation and Conclusions. Age, gender and vitamin status influence both FtHCy and PML-tHCy in normal subjects. Reference values should be calculated according to age and sex. (C) 2002, Ferrata Storti Foundation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
