The mustard trypsin inhibitor 2 (MTI2) was expressed in Escherichia coli. A specific procedure for its production and purification is described. The recombinant protein was recovered from the insoluble fraction of protein extracts, then renaturated and purified by ion-exchange and gel filtration chromatography. Finally the inhibitory activity was determined against trypsin.
Stefan A., Ugolini L., Martelli E., Palmieri S., Hochkoeppler A. (2009). Expression and purification of the recombinant mustard trypsin inhibitor 2 (MTI2) in Escherichia coli. JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108(4), 282-285 [10.1016/j.jbiosc.2009.04.016].
Expression and purification of the recombinant mustard trypsin inhibitor 2 (MTI2) in Escherichia coli
STEFAN, ALESSANDRA;HOCHKOEPPLER, ALEJANDRO
2009
Abstract
The mustard trypsin inhibitor 2 (MTI2) was expressed in Escherichia coli. A specific procedure for its production and purification is described. The recombinant protein was recovered from the insoluble fraction of protein extracts, then renaturated and purified by ion-exchange and gel filtration chromatography. Finally the inhibitory activity was determined against trypsin.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
