The mustard trypsin inhibitor 2 (MTI2) was expressed in Escherichia coli. A specific procedure for its production and purification is described. The recombinant protein was recovered from the insoluble fraction of protein extracts, then renaturated and purified by ion-exchange and gel filtration chromatography. Finally the inhibitory activity was determined against trypsin.

Expression and purification of the recombinant mustard trypsin inhibitor 2 (MTI2) in Escherichia coli

STEFAN, ALESSANDRA;HOCHKOEPPLER, ALEJANDRO
2009

Abstract

The mustard trypsin inhibitor 2 (MTI2) was expressed in Escherichia coli. A specific procedure for its production and purification is described. The recombinant protein was recovered from the insoluble fraction of protein extracts, then renaturated and purified by ion-exchange and gel filtration chromatography. Finally the inhibitory activity was determined against trypsin.
Stefan A.; Ugolini L.; Martelli E.; Palmieri S.; Hochkoeppler A.
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/78520
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus 1
  • ???jsp.display-item.citation.isi??? 1
social impact