The stick-insect genus Pijnackeria includes four diploid bisexual and two polyploid (3n, 4n) parthenogenetic species. Earlier analyses of the tetraploid parthenogen P. hispanica using mitochondrial markers allowed tracing its maternal ancestry to Pijnackeria originis, while no maternal nuclear contribution was found, thus suggesting an androgenetic and hybrid origin. The recently described Pijnackeria recondita—showing, among other features, a specific antennal structure linking it to the tetraploid parthenogen—prompted us to check whether the new species could be the unknown paternal ancestor of P. hispanica. In this work, we use karyology and molecular analysis of the mitochondrial gene cytochrome c oxidase subunit 2 (cox2) and the nuclear gene elongation factor 1 subunit α (ef1-α) to investigate the origin of such a complex tetraploid hybrid parthenogen. The molecular analysis supported P. recondita as being a paternal ancestor of P. hispanica, but also suggested that two more fathering species have to be taken into account: P. barbarae and the unknown paternal ancestor of the triploid hybrid P. masettii. Therefore, P. hispanica is apparently a polyphyletic chimeric androgen, which we propose to indicate as an androgenetic complex. Our data also revealed that P. hispanica is between 1.96 Myr and 3.31 Myr old, making it the oldest parthenogenetic taxon discovered among insects.

Milani L., Scali V., Punzi E., Luchetti A., Ghiselli F. (2020). The puzzling taxonomic rank of Pijnackeria hispanica, a chimerical hybrid androgen (Insecta, Phasmida). ORGANISMS DIVERSITY & EVOLUTION, 20, 285-297 [10.1007/s13127-020-00436-1].

The puzzling taxonomic rank of Pijnackeria hispanica, a chimerical hybrid androgen (Insecta, Phasmida)

Milani L.
;
Scali V.
;
Punzi E.;Luchetti A.;Ghiselli F.
2020

Abstract

The stick-insect genus Pijnackeria includes four diploid bisexual and two polyploid (3n, 4n) parthenogenetic species. Earlier analyses of the tetraploid parthenogen P. hispanica using mitochondrial markers allowed tracing its maternal ancestry to Pijnackeria originis, while no maternal nuclear contribution was found, thus suggesting an androgenetic and hybrid origin. The recently described Pijnackeria recondita—showing, among other features, a specific antennal structure linking it to the tetraploid parthenogen—prompted us to check whether the new species could be the unknown paternal ancestor of P. hispanica. In this work, we use karyology and molecular analysis of the mitochondrial gene cytochrome c oxidase subunit 2 (cox2) and the nuclear gene elongation factor 1 subunit α (ef1-α) to investigate the origin of such a complex tetraploid hybrid parthenogen. The molecular analysis supported P. recondita as being a paternal ancestor of P. hispanica, but also suggested that two more fathering species have to be taken into account: P. barbarae and the unknown paternal ancestor of the triploid hybrid P. masettii. Therefore, P. hispanica is apparently a polyphyletic chimeric androgen, which we propose to indicate as an androgenetic complex. Our data also revealed that P. hispanica is between 1.96 Myr and 3.31 Myr old, making it the oldest parthenogenetic taxon discovered among insects.
2020
Milani L., Scali V., Punzi E., Luchetti A., Ghiselli F. (2020). The puzzling taxonomic rank of Pijnackeria hispanica, a chimerical hybrid androgen (Insecta, Phasmida). ORGANISMS DIVERSITY & EVOLUTION, 20, 285-297 [10.1007/s13127-020-00436-1].
Milani L.; Scali V.; Punzi E.; Luchetti A.; Ghiselli F.
File in questo prodotto:
File Dimensione Formato  
Milani-et-al_2020.pdf

accesso aperto

Tipo: Postprint
Licenza: Licenza per accesso libero gratuito
Dimensione 3.49 MB
Formato Adobe PDF
3.49 MB Adobe PDF Visualizza/Apri
Suppl_Figure1.pdf

accesso aperto

Tipo: File Supplementare
Licenza: Licenza per accesso libero gratuito
Dimensione 94.21 kB
Formato Adobe PDF
94.21 kB Adobe PDF Visualizza/Apri
Suppl-material1_Milani_et_al_Genbank-acc-nos.xlsx

accesso aperto

Tipo: File Supplementare
Licenza: Licenza per accesso libero gratuito
Dimensione 13.43 kB
Formato Microsoft Excel XML
13.43 kB Microsoft Excel XML Visualizza/Apri
Suppl-material2_Milani_et_al_cox2.pdf

accesso aperto

Tipo: File Supplementare
Licenza: Licenza per accesso libero gratuito
Dimensione 40.83 kB
Formato Adobe PDF
40.83 kB Adobe PDF Visualizza/Apri
Suppl-material3_Milani_et_al_ef1a.pdf

accesso aperto

Tipo: File Supplementare
Licenza: Licenza per accesso libero gratuito
Dimensione 31.45 kB
Formato Adobe PDF
31.45 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/781529
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 1
  • ???jsp.display-item.citation.isi??? 1
social impact