We report that the efficiency of reprogramming human somatic cells to induced pluripotent stem cells (hiPSCs) can be dramatically improved in a microfluidic environment. Microliter-volume confinement resulted in a 50-fold increase in efficiency over traditional reprogramming by delivery of synthetic mRNAs encoding transcription factors. In these small volumes, extracellular components of the TGF-b and other signaling pathways exhibited temporal regulation that appears critical to acquisition of pluripotency. The high quality and purity of the resulting hiPSCs (micro-hiPSCs) allowed direct differentiation into functional hepatocyte- and cardiomyocyte-like cells in the same platform without additional expansion.
High-efficiency cellular reprogramming with microfluidics / LUNI, CAMILLA; GIULITTI, STEFANO; SERENA, ELENA; Ferrari, Luca; ZAMBON, ALESSANDRO; GAGLIANO, ONELIA; GIOBBE, GIOVANNI GIUSEPPE; MICHIELIN, FEDERICA; Knöbel, Sebastian; Bosio, Andreas; ELVASSORE, NICOLA. - In: NATURE METHODS. - ISSN 1548-7091. - ELETTRONICO. - 13:5(2016), pp. 446-452. [10.1038/nmeth.3832]
High-efficiency cellular reprogramming with microfluidics
LUNI, CAMILLA;ZAMBON, ALESSANDRO;
2016
Abstract
We report that the efficiency of reprogramming human somatic cells to induced pluripotent stem cells (hiPSCs) can be dramatically improved in a microfluidic environment. Microliter-volume confinement resulted in a 50-fold increase in efficiency over traditional reprogramming by delivery of synthetic mRNAs encoding transcription factors. In these small volumes, extracellular components of the TGF-b and other signaling pathways exhibited temporal regulation that appears critical to acquisition of pluripotency. The high quality and purity of the resulting hiPSCs (micro-hiPSCs) allowed direct differentiation into functional hepatocyte- and cardiomyocyte-like cells in the same platform without additional expansion.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
