A twin-column Multicolumn Countercurrent Solvent Gradient Purifica-tion (MCSGP) process has been developed for the purification of a ther-apeutic peptide, glucagon, from a crude synthetic mixture. This semi-continuous process uses two identical columns operating either in inter-connected or in batch mode, thus enabling the internal recycle of the por-tions of the eluting stream which do not comply with purity specifications.Because of this feature, which actually results in the simulated countercur-rent movement of the stationary phase with respect to the mobile one, theyield-purity trade-off typical of traditional batch preparative chromatog-raphy can be alleviated. Moreover, the purification process can be com-pletely automatized

De Luca, C., Felletti, S., Lievore, G., Buratti, A., Vogg, S., Morbidelli, M., et al. (2020). From batch to continuous chromatographic purification of a therapeutic peptide through Multicolumn Countercurrent Solvent Gradient Purification (MCSGP). JOURNAL OF CHROMATOGRAPHY A, 1625, 461304-461304 [10.1016/j.chroma.2020.461304].

From batch to continuous chromatographic purification of a therapeutic peptide through Multicolumn Countercurrent Solvent Gradient Purification (MCSGP)

Cabri, W.
2020

Abstract

A twin-column Multicolumn Countercurrent Solvent Gradient Purifica-tion (MCSGP) process has been developed for the purification of a ther-apeutic peptide, glucagon, from a crude synthetic mixture. This semi-continuous process uses two identical columns operating either in inter-connected or in batch mode, thus enabling the internal recycle of the por-tions of the eluting stream which do not comply with purity specifications.Because of this feature, which actually results in the simulated countercur-rent movement of the stationary phase with respect to the mobile one, theyield-purity trade-off typical of traditional batch preparative chromatog-raphy can be alleviated. Moreover, the purification process can be com-pletely automatized
2020
De Luca, C., Felletti, S., Lievore, G., Buratti, A., Vogg, S., Morbidelli, M., et al. (2020). From batch to continuous chromatographic purification of a therapeutic peptide through Multicolumn Countercurrent Solvent Gradient Purification (MCSGP). JOURNAL OF CHROMATOGRAPHY A, 1625, 461304-461304 [10.1016/j.chroma.2020.461304].
De Luca, C; Felletti, S ; Lievore, G; Buratti, A; Vogg, S; Morbidelli, M; Cavazzini, A; Catani, M; Macis, M; Ricci, A; Cabri, W.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/767786
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