The renaissance of peptides as prospective therapeutics has fostered the development of novel strategies for their synthesis and modification. In this context, besides the developmentofnewchemicalpeptideligationapproaches,especiallytheuseofenzymes as a versatile tool has gained increased attention. Nowadays, due to their inherent properties such as excellent regio- and chemoselectivity, enzymes represent invaluable instruments in both academic and industrial laboratories. This mini-review focuses on natural- and engineered peptide ligases that can form a new peptide (amide) bond between the C-terminal carboxy and N-terminal amino group of a peptide and/or protein. The pro’s and cons of several enzyme classes such as Sortases, Asparaginyl Endoproteases, Trypsin relatedenzymesand as a centralfocus subtilisin-derived variants are summarized. Most recent developments with regards to ligation and cyclization are highlighted
Natural Occurring and Engineered Enzymes for Peptide Ligation and Cyclization
Cabri W
2019
Abstract
The renaissance of peptides as prospective therapeutics has fostered the development of novel strategies for their synthesis and modification. In this context, besides the developmentofnewchemicalpeptideligationapproaches,especiallytheuseofenzymes as a versatile tool has gained increased attention. Nowadays, due to their inherent properties such as excellent regio- and chemoselectivity, enzymes represent invaluable instruments in both academic and industrial laboratories. This mini-review focuses on natural- and engineered peptide ligases that can form a new peptide (amide) bond between the C-terminal carboxy and N-terminal amino group of a peptide and/or protein. The pro’s and cons of several enzyme classes such as Sortases, Asparaginyl Endoproteases, Trypsin relatedenzymesand as a centralfocus subtilisin-derived variants are summarized. Most recent developments with regards to ligation and cyclization are highlightedI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.